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Human Obestatin ELISA Kit

  • 中文名称:
    人肥胖抑制素酶联免疫试剂盒
  • 货号:
    CSB-E12818h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO人肥胖抑制素(Obestatin)酶联免疫检测试剂盒(货号:CSB-E12818h),采用双抗体夹心法定量分析血清、血浆、细胞培养上清液、尿液及组织匀浆样本中Obestatin含量,检测灵敏度为4.69 pg/mL,线性范围覆盖4.69-300 pg/mL。肥胖抑制素是由胃部分泌的肽类激素,参与调节食欲、能量代谢及胃肠道功能,其与生长激素促分泌素受体的相互作用在体重调控机制中具有研究价值。本试剂盒适用于体外科研场景,例如探究肥胖相关代谢疾病机制、胃肠功能调控或内分泌信号通路研究,研究者可通过标准曲线精确测定样本中Obestatin的动态浓度变化。试剂盒操作流程包含预包被特异性抗体的微孔板、生物素标记检测抗体及链霉亲和素-HRP系统,支持在3-4小时内完成孵育与显色步骤,配套提供标准品、缓冲液及终止液,实验数据稳定可靠,为体外实验提供高效检测方案。
  • 缩写:
    Obestatin
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 检测范围:
    4.69 pg/mL-300 pg/mL
  • 灵敏度:
    1.17 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess. 
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess. 
     
  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human Obestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

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  • 回收率:

    The recovery of human Obestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

  • 数据处理:
  • 货期:
    3-5 working days

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