Human Neuropeptide-like protein C4orf48(C4orf48) ELISA kit

Human Neuropeptide-like protein C4orf48(C4orf48) ELISA kit

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中文名称：

人神经肽样蛋白C4orf48(C4orf48)酶联免疫试剂盒
货号：

CSB-EL003989HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人神经肽样蛋白C4orf48（C4orf48）酶联免疫检测试剂盒（货号：CSB-EL003989HU），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中C4orf48蛋白的表达水平。C4orf48是定位于人类第4号染色体开放阅读框的基因编码蛋白，其结构特征提示其可能参与神经肽介导的信号调控或细胞间相互作用，但具体生物学功能尚需进一步研究。该试剂盒检测灵敏度达0.625 ng/mL，线性范围为0.625-40 ng/mL，可精准捕捉样本中目标蛋白的浓度变化。实验操作流程标准化，配备预包被特异性捕获抗体和生物素标记检测抗体系统，通过显色反应实现靶蛋白定量分析。适用于基础研究领域探索C4orf48在神经系统发育、神经退行性疾病模型中的表达调控，或结合组织样本分析其区域性分布特征。科研人员可通过检测体液或组织裂解液中的蛋白含量，研究其在特定生理病理过程中的潜在作用机制，为神经生物学研究提供实验工具。
别名：

C4orf48 ELISA Kit; Chr4_55 ELISA Kit; Neuropeptide-like protein C4orf48 ELISA Kit
缩写：

C4orf48
Uniprot No.：

Q5BLP8
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	87-98
1:8	Average %	103
	Range %	97-108

回收率：

The recovery of human C4orf48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	81-92
EDTA plasma (n=4)	102	97-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.564	2.689	2.627	2.494
20	2.185	2.077	2.131	1.998
10	1.459	1.524	1.492	1.359
5	0.812	0.828	0.820	0.687
2.5	0.423	0.432	0.428	0.295
1.25	0.283	0.294	0.289	0.156
0.625	0.186	0.192	0.189	0.056
0	0.132	0.134	0.133

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The secreted micropeptide C4orf48 enhances renal fibrosis via an RNA-binding mechanism J Yang,The Journal of clinical investigation,2024

亚细胞定位：

Secreted.
组织特异性：

Brain-specific (at protein level).
数据库链接：

HGNC: 34437

OMIM: 614690

STRING: 9606.ENSP00000386807

UniGene: Hs.446474

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.