Human NGAL/MMP-9 complex ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专用于定量检测人血清、血浆、唾液、细胞培养上清及组织匀浆样本中的中性粒细胞明胶酶相关脂质运载蛋白（NGAL）与基质金属蛋白酶9（MMP-9）形成的复合物。NGAL/MMP-9复合物在炎症反应、组织修复及肿瘤微环境调控中发挥重要作用，其表达水平与急性肾损伤、癌症进展等病理过程密切相关。试剂盒检测范围为0.313-20 ng/mL，灵敏度达0.313 ng/mL，可在4-5小时内完成检测，适用于基础医学研究中疾病机制探索、生物标志物分析及药物干预效果评估。提供的预包被板条、标准品及检测试剂经过严格验证，支持多种生物样本类型检测，为炎症相关疾病模型构建、肿瘤微环境研究及肾脏功能损伤实验提供高效工具。实验操作需配合酶标仪进行450nm波长吸光度测定，通过标准曲线计算目标物浓度，建议实验过程中同步进行质量控制样本检测以确保数据可靠性。
缩写：

NGAL/MMP-9 complex
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva, cell culture supernates, tissue homogenates
检测范围：

0.313 ng/mL-20 ng/mL
灵敏度：

0.029 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	91
Range %	87-98
1:20	Average %	95
Range %	90-102
1:40	Average %	93
Range %	87-99
1:80	Average %	94
Range %	86-105

回收率：

The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	94	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.