Human N-terminal procollagen Ⅳ propeptide,PⅣNP ELISA Kit

产品详情

产品描述：

人Ⅳ型前胶原氨基末端肽(PⅣNP)酶联免疫试剂盒（CSB-EQ027190HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PⅣNP含量。试剂盒检测范围为2.8 ng/mL-180 ng/mL，常用于肝纤维化、肺纤维化等疾病模型的机制研究，适用于体外培养体系、动物实验样本中胶原合成活性的动态监测，可为纤维化相关分子机制探索、药物干预效果评估等研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N-terminal procollagen 4 propeptide,P4NP,_x000D_ N-terminal procollagen IV propeptide,PIVNP
缩写：

PⅣNP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.8 ng/mL-180 ng/mL
灵敏度：

0.7 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PIVNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	99
	Range %	87-104
1:10	Average %	105
	Range %	100-110
1:20	Average %	93
	Range %	86-97
1:40	Average %	104
	Range %	95-107

回收率：

The recovery of human PIVNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	100-111
EDTA plasma (n=4)	101	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.