Human Myeloid Progenitor Inhibitory Factor 1,MPIF-1 ELISA Kit

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中文名称：

人骨髓抑制因子1(MPIF-1/CCL23)酶联免疫试剂盒
货号：

CSB-E07309h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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本试剂盒专用于定量检测人血清、血浆及组织匀浆样本中的骨髓抑制因子1（MPIF-1/CCL23），该靶标属于CC趋化因子家族成员，在炎症反应、免疫调控及细胞迁移等生理病理过程中发挥重要作用。产品基于双抗体夹心法原理设计，预包被高特异性抗体可精准捕获目标蛋白，通过酶标抗体复合物显色反应实现定量分析，检测灵敏度达39 pg/mL，线性范围覆盖39-2500 pg/mL，满足低丰度样本的检测需求。适用于科研领域中对MPIF-1/CCL23表达水平的动态监测，例如探究其在自身免疫性疾病、慢性炎症或肿瘤微环境中的调控机制，为免疫相关疾病的基础研究提供可靠工具。试剂盒包含优化后的预包被板、标准品、检测抗体及全套反应试剂，操作流程标准化，兼容常规酶标仪读数，可高效完成批量样本分析。
别名：

C C motif chemokine ligand 23 ELISA Kit; C-C motif chemokine 23 ELISA Kit; C6 beta chemokine ELISA Kit; CCL 23 ELISA Kit; CCL23 ELISA Kit; CCL23(30-99) ELISA Kit; CCL23_HUMAN ELISA Kit; Chemokine (C C motif) ligand 23 ELISA Kit; Chemokine CC motif ligand 23 ELISA Kit; ck beta 8 ELISA Kit; CK-beta-8 ELISA Kit; CKB 8 ELISA Kit; CKB-8 ELISA Kit; Ckb-8-1 ELISA Kit; CKB8 ELISA Kit; Hmrp-2a ELISA Kit; Macrophage inflammatory protein 3 ELISA Kit; MIP 3 ELISA Kit; MIP-3 ELISA Kit; MIP3 ELISA Kit; MPIF 1 ELISA Kit; MPIF-1 ELISA Kit; MPIF1 ELISA Kit; Myeloid progenitor inhibitory factor 1 ELISA Kit; OTTHUMP00000163948 ELISA Kit; SCYA 23 ELISA Kit; SCYA23 ELISA Kit; Small inducible cytokine A 23 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys); member 23 ELISA Kit; Small inducible cytokine subfamily A; member 23 ELISA Kit; Small-inducible cytokine A23 ELISA Kit
缩写：

CCL23
Uniprot No.：

P55773
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

39 pg/mL-2500 pg/mL
灵敏度：

9.7 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	309.863	319.761	329.703	315.796	325.721	335.691
SD	0.035	0.043	0.048	0.038	0.045	0.064
CV(%)	4.353	5.218	5.687	4.657	5.383	7.477

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MPIF-1/CCL23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-96

1:2

Average %

Range %

90-97

1:4

Average %

100

Range %

93-109

1:8

Average %

Range %

85-96

回收率：

The recovery of human MPIF-1/CCL23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	95	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.820	2.732	2.776	2.672
1250	2.057	2.036	2.047	1.943
625	1.306	1.291	1.299	1.195
312.5	0.851	0.796	0.824	0.720
156	0.551	0.531	0.541	0.437
78	0.372	0.358	0.365	0.261
39	0.258	0.249	0.254	0.150
0	0.108	0.099	0.104
LOD 9.7 pg/ml

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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最新研究进展：

CCL23是一种趋化因子，已被证明在多种疾病中发挥重要作用，如哮喘、肺癌和自身免疫性疾病等。研究表明，CCL23在哮喘和COPD患者中的含量显著增加，并且与疾病的严重程度呈正相关。此外，最近的研究表明，CCL23可以促进肿瘤细胞增殖和转移，因此可能是治疗肿瘤的潜在靶点。
功能：

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
基因功能参考文献：
1. CCL23 is posttranslationally modified by trypsin-like serine proteases and CCL23(47-117) might be a major active form of CCL23 in nasal polyps with chronic rhinosinusitis. PMID: 26560043
2. The coexistence of diabetes and oxidative stress independently affected CCL23 levels, while the presence of Cardiovascular disease and inflammation had no impact on its concentrations. PMID: 24995525
3. ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities PMID: 24384427
4. Data show CCL23 stimulates chemotaxis of human THP-1 monocytes and enhances release of MMP-2, and suggest it plays a role in atherogenesis. PMID: 21656154
5. Overproduction of CCL23 in nasal polyps might contribute to the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps PMID: 21497884
6. Patients with systemic sclerosis who had elevated CCL23 levels had shorter disease duration, and a higher frequency of pulmonary arterial hypertension. Serum CCL23 level was increased in early phase of disease and could be a marker for disease activity. PMID: 20824279
7. Fresh eosinophils contained trace amounts of CCL23 protein. CCL23 was significantly released into the supernatant when the eosinophils were stimulated with GM-CSF or IL-5 but not with IFN-gamma or immobilized sIgA. PMID: 21646793
8. independently associated with coronary atherosclerosis PMID: 20187767
9. CKbeta8 transduces the chemotaxis signal through the G(i)/G(o) protein, phospholipase C, protein kinase C delta and NF-kappaB. PMID: 19951712
10. CCL23 promoted chemotactic migration and differentiation of endothelial cells, and neovascularization in the chick chorioallantoic membrane PMID: 15927850
11. May play a direct role in angiogenesis via upregulation of matrix metalloproteinase (MMP)-2 expression. PMID: 16378600
12. Collectively, these data suggest a link between the inducible phenotype of CCL23 expression in monocytes by the prototype Th2 molecule pair IL-4/STAT6 and the increased number of CCL23-expressing cells in skin of atopic dermatitis patients. PMID: 17371990
13. A peptide ligand termed SHAAGtide is cleaved from CCL23; is itself an attractant of monocytes and neutrophils in vitro; recruits leukocytes in vivo; is more potent than other natural agents with activity for FPRL1; receives authors' designation "CCR12." PMID: 17513790
14. This protein may participate in the malignant progression of certain human cancer cells that overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. PMID: 18258606
15. CCL23, M-CSF, TNFRSF9, TNF-alpha, and CXCL13 are predictive of rheumatoid arthritis disease activity and may be useful in the definition of disease subphenotypes and in the measurement of response to therapy in clinical studies. PMID: 18668547
16. these findings suggest that CCL23 results in up-regulation of KDR/flk-1 receptor gene transcription and protein expression and that KDR/Flk-1 up-regulation induced by CCL23 may contribute to potentiation of VEGF action in angiogenesis. PMID: 19265684
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
组织特异性：

High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CC
数据库链接：

HGNC: 10622

OMIM: 602494

KEGG: hsa:6368

UniGene: Hs.169191

Human Myeloid Progenitor Inhibitory Factor 1,MPIF-1 ELISA Kit

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