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  4. Human Mucin-15(MUC15) ELISA kit

Human Mucin-15(MUC15) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人粘蛋白-15(MUC15)酶联免疫试剂盒
  • 货号：
    CSB-EL015218HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人粘蛋白-15（MUC15）酶联免疫试剂盒（货号CSB-EL015218HU）基于双抗体夹心法定量检测血清、血浆、组织匀浆及细胞裂解液中的MUC15蛋白浓度，检测灵敏度达125 pg/mL，线性范围为125-8000 pg/mL。MUC15属于跨膜型粘蛋白家族成员，在维持上皮屏障功能、细胞粘附及信号转导中发挥重要作用，其异常表达与肿瘤微环境调控、免疫应答等生物学过程密切相关。CUSABIO试剂盒采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立定量曲线，结合HRP酶联显色系统实现精准检测，实验全程约需4.5小时，样本用量仅需100μL。该产品适用于科研领域对MUC15蛋白表达水平的定量分析，例如在癌症发生机制、炎症相关通路研究或组织特异性表达谱分析中评估生物样本中MUC15的动态变化，为探索其在细胞通讯、疾病生物标志物筛选等基础研究提供可靠工具。试剂盒配套提供标准品、检测抗体及实验所需缓冲液组分，需自备酶标仪进行吸光度测定。
  • 别名：
    Component II ELISA Kit; Glycoprotein 4 ELISA Kit; Glycoprotein C ELISA Kit; MUC 15 ELISA Kit; MUC-15 ELISA Kit; Muc15 ELISA Kit; MUC15_HUMAN ELISA Kit; Mucin 15 ELISA Kit; Mucin 15 cell surface associated ELISA Kit; Mucin-15 ELISA Kit; PAS3 ELISA Kit; PASIII ELISA Kit
  • 缩写：
    MUC15
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MUC15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 89-103
    1:2 Average % 95
    Range % 90-99
    1:4 Average % 97
    Range % 87-102
    1:8 Average % 103
    Range % 97-108
  • 回收率：
    The recovery of human MUC15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 81-90
    EDTA plasma (n=4) 92 88-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 1.972 2.027 2.000 1.863
    4000 1.456 1.436 1.446 1.309
    2000 0.902 0.925 0.914 0.777
    1000 0.576 0.583 0.580 0.443
    500 0.430 0.421 0.426 0.289
    250 0.317 0.309 0.313 0.176
    125 0.250 0.242 0.246 0.109
    0 0.140 0.134 0.137 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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MUC15 antibodies

MUC15 Proteins

靶点详情

  • 功能：
    May play a role in the cell adhesion to the extracellular matrix.
  • 基因功能参考文献：
    1. Frameshift mutations of MUC15 gene is associated with gastric and colorectal cancers. PMID: 25573589
    2. Multivariate analysis suggested that MUC15 overexpression was an independent factor for prognosis (hazard risk: 3.216; P=0.009). It was concluded that MUC15 is overexpressed in glioma tissues. PMID: 24710941
    3. MUC15 inhibits dimerization of EGFR and PI3K-AKT signaling and is associated with aggressive hepatocellular carcinomas in patients. PMID: 23933603
    4. MUC15 is a strong candidate for eczema. PMID: 22657408
    5. MUC4 and MUC15 were overexpressed in papillary thyroid carcinoma, and high MUC15 expression was associated with high malignant potential. PMID: 21615302
    6. MUC15 was expressed most highly in human placenta. MUC15 mRNA & protein increased with gestational age (P < 0.05, 1st versus 3rd trimester). Differential expression of MUC15 in human placentas may play critical role in regulation of trophoblast invasion. PMID: 17720698
    7. Overexpression of MUC15 activates extracellular signal-regulated kinase 1/2 and promotes the oncogenic potential of colon cancer cells. PMID: 19520792

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
  • 组织特异性：
    Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, bone marrow, lymph node and lung.
  • 数据库链接：

    HGNC: 14956

    OMIM: 608566

    KEGG: hsa:143662

    STRING: 9606.ENSP00000416753

    UniGene: Hs.407152

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |