Human Monoglyceride lipase(MGLL) ELISA kit

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.

1. Using a multimethod approach, the authors show that the dynamically relevant Trp-289 and Leu-232 residues serve as communication hubs within an allosteric protein network that controls signal propagation to the active site, and thus, regulates active-inactive interconversion of human MGL. PMID: 29379013
2. RNA interference, specific pharmacological inhibitor JZL-184 and gene knock-in of MAGL were utilized to investigate the effects of MAGL on hepatocellular carcinoma (HCC) cell proliferation, apoptosis, and invasion. MAGL played important roles in both proliferation and invasion of HCC cells. PMID: 27767105
3. This review summarizes the basics of monoglyceride metabolism and provides an overview on the therapeutic potential of MGL. [Review] PMID: 28213089
4. MGLL may have a role in progression of gastrointestinal stromal tumors PMID: 27366945
5. the upregulation of MAGL in hepatocellular carcinoma cells promoted cell growth and invasiveness abilities, and mediated epithelial-mesenchymal transition PMID: 27884159
6. we clarify the key role of Phe159 and Ile179, two conserved residues within the lid domain, in regulating substrate specificity in MAGL. We conclude by proposing that other structurally related lipases may share this lid-domain-mediated mechanism for substrate specificity. PMID: 28088576
7. the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes, was investigated. PMID: 26948343
8. there was evidence that MGLL rs604300 genotype interacts with early life adversity to predict threat-related basolateral amygdala habituation, a neural phenotype linked to the endocannabinoid system and addiction PMID: 26595473
9. This study unravels a novel mechanism of SND1 function and identifies MGLL as a unique tumor suppressor for HCC. MGLL might function as a homeostatic regulator of Akt restraining its activation. PMID: 26997225
10. The data highlight specific inter-residue interactions within hMGL PMID: 26555264
11. Monoacylglycerol lipase sulfenylation might act as an intrinsic neuroprotective mechanism by potentiating 2-AG signaling at CB1 receptors. PMID: 26000748
12. Molecular dynamics and nudged elastic band simulations were used to explore the conformational transition pathway of the helix alpha4 of human monoacylglycerol lipase. PMID: 25078047
13. role of monoacylglycerol lipase (MAGL) in the cancer progress PMID: 24633487
14. Our findings establish that MAGL promotes metastases in nasopharyngeal carcinoma PMID: 25120746
15. In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence. PMID: 24616451
16. In obese humans, FAAH or MGL activity in adipocytes is not affected by diabetes, dyslipidaemia or other markers of metabolic dysfunction. PMID: 24593280
17. Mutation of Cys242 was also found to impair inhibition of monoacylglycerol lipase. PMID: 24368842
18. MGL interaction with a phospholipid membrane bilayer induces regional changes in the enzyme's conformation that favor its recruiting lipophilic substrate from membrane stores to the active site resulting in enhanced MGL catalytic activity. PMID: 23553709
19. Data indicate that N,N-dimethyl-5-(4-phenoxyphenyl)-2H-tetrazole-2-carboxamide inhibited monoacylglycerol lipase (MAGL) with IC50 0.028 muM. PMID: 23455058
20. MGL expression declines after peaking in infancy. PMID: 22827915
21. results suggest that MGL plays a negative regulatory role in phosphatidylinositol-3 kinase/Akt signaling and tumor cell growth PMID: 22349814
22. No association was found between our inflammatory bowel disease cohort and the candidate single nucleotide polymorphisms for MGL (CD/HC: P=0.37 and UC/HC: P=0.25). PMID: 22664939
23. MAGL is elevated in androgen-independent versus androgen-dependent human prostate cancer cell lines, and pharmacological or RNA-interference disruption of this enzyme impairs prostate cancer aggressiveness. PMID: 21802006
24. High MAGL is associated with colorectal cancer. PMID: 21543155
25. Data present a high-resolution structure of a ligand-bound, soluble form of human monoglyceride lipase. PMID: 21308848
26. One interval in the FAAH promoter and three intervals in the MGLL gene were associated with high BMI. PMID: 21118518
27. Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid in the brain and is believed to be hydrolyzed primarily by serine hydrolase monoacylglycerol lipase (MAGL) in a transgenic mouse model with targeted disruption of MAGL. PMID: 20855465
28. Identification of an active site hydrogen bond network in human monoacylglycerol lipase. PMID: 20464001
29. An apolar helix covering the active site also gives structural insight into the amphitropic character of MAGL, and likely explains how MAGL interacts with membranes to recruit its substrate. PMID: 19957260
30. MGL shares the classic fold of the alpha/beta hydrolase family but depicts an unusually large hydrophobic occluded tunnel with a highly flexible lid at its entry and the catalytic triad buried at its end. PMID: 19962385
31. Study shows that monoacylglycerol lipase (MAGL) is highly expressed in aggressive cancer cells and primary tumors; it regulates a fatty acid network enriched in oncogenic signaling lipids that promotes migration, invasion, survival, and tumor growth. PMID: 20079333
32. not associated with susceptibility to alcoholism in a Japanese population. PMID: 17621164
33. full proteomic characterization of hMGL was carried out, which showed (1) an absence of intramolecular disulfide bridges in the functional, recombinant enzyme and (2) the post-translational removal of the enzyme's N-terminal methionine PMID: 18452279
34. Identification of amino acids critical to the catalytic activity PMID: 18721756
35. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. PMID: 19233690

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Cytoplasm, cytosol. Membrane; Peripheral membrane protein.

AB hydrolase superfamily, Monoacylglycerol lipase family

Detected in adipose tissue, lung, liver, kidney, brain and heart.

HGNC: 17038

OMIM: 609699

KEGG: hsa:11343

STRING: 9606.ENSP00000265052

UniGene: Hs.277035