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  4. Human Microfibrillar-associated protein 5(MFAP5) ELISA kit

Human Microfibrillar-associated protein 5(MFAP5) ELISA kit

说明书下载
  • 中文名称：
    人微纤丝关联蛋白5(MFAP5)酶联免疫试剂盒
  • 货号：
    CSB-EL013750HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人微纤丝关联蛋白5(MFAP5)酶联免疫试剂盒（CSB-EL013750HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MFAP5含量。MFAP5 即微原纤维相关蛋白 5，属于微原纤维相关蛋白家族成员。其与弹性纤维形成、细胞黏附等过程相关。研究机制方面，它在多种疾病如肿瘤、纤维化疾病中异常表达，可能通过影响细胞外基质等发挥作用，是潜在的疾病治疗靶点。试剂盒检测范围为12.5pg/ml- 800pg/ml，适用于心血管疾病机制研究、肿瘤微环境分析、组织再生能力评估等科研领域，适用于体外研究场景，可为探索MFAP5在细胞外基质重塑、疾病生物标志物筛选等方向提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AAT9 ELISA Kit; MAGP 2 ELISA Kit; MAGP-2 ELISA Kit; MAGP2 ELISA Kit; MFAP 5 ELISA Kit; MFAP-5 ELISA Kit; MFAP5 ELISA Kit; MFAP5_HUMAN ELISA Kit; Microfibril associated glycoprotein 2 ELISA Kit; Microfibril-associated glycoprotein 2 ELISA Kit; microfibrillar associated protein 5 ELISA Kit; Microfibrillar associated protein 5 precursor ELISA Kit; Microfibrillar-associated protein 5 ELISA Kit; MP25 ELISA Kit
  • 缩写：
    MFAP5
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5pg/ml - 800pg/ml
  • 灵敏度：
    3.125pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MFAP5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    97

    Range %

    92-103

    1:2

    Average %

    91

    Range %

    82-97

    1:4

    Average %

    103

    Range %

    99-109

    1:8

    Average %

    87

    Range %

    82-97

  • 回收率：

    The recovery of human MFAP5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    85-99

    EDTA plasma (n=4)

    111

    105-116

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    800

    2.591

    2.612

    2.602

    2.461

    400

    2.247

    2.251

    2.249

    2.108

    200

    1.630

    1.655

    1.643

    1.502

    100

    1.085

    1.074

    1.080

    0.939

    50

    0.654

    0.698

    0.676

    0.535

    25

    0.396

    0.401

    0.399

    0.258

    12.5

    0.263

    0.245

    0.254

    0.113

    0

    0.144

    0.137

    0.141

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

MFAP5 antibodies

MFAP5 Proteins

靶点详情

  • 功能：
    May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity. Component of the elastin-associated microfibrils.
  • 基因功能参考文献：
    1. MFAP5 was up-regulated in breast cancers compared with that in normal breast tissues, and further increased in breast cancer bone metastasis. Functionally, MFAP5 overexpression accelerated breast cancer cell proliferation and migration, while an opposite effect was observed when MFAP5 was knocked down. PMID: 29526753
    2. Study shows that over-expression of MFAP5 and TNNC1 is correlated with cervical lymph node metastasis (CLNM), metastasis relapse-free survival and overall survival. These results propose that MFAP5 and TNNC1 may be potential markers for predicting occult cervical lymphatic metastasis and prognosis of oral tongue carcinoma. PMID: 27713166
    3. Likely pathogenic variants included a TGFB2 variant in one patient and a SMAD3 variant in another. These variants have been reported previously in individuals with similar phenotypes. Variants of uncertain significance of particular interest included novel variants in MYLK and MFAP5, which were identified in a third patient PMID: 26854089
    4. The results answer the question of how MAGP2 controls cell type dependent Notch signaling, but more importantly uncover a new mechanism to understand how extracellular matrices and cellular environments impact Notch signaling. PMID: 26808411
    5. Our results demonstrate that factors involving low-grade inflammation modulate MFAP5 expression and that the modified expression of MFAP5 may further regulate adipose tissue inflammation. PMID: 26054006
    6. FAK/CREB/TNNC1 has a role in mediating the effect of stromal MFAP5 on ovarian cancer metastatic potential PMID: 25277212
    7. Alteration of MAGP-2, a component of microfibrils and elastic fibers, appears as an initiating mechanism of inherited Thoracic aortic aneurysm and dissection (TAAD). PMID: 25434006
    8. EPS led to the discovery of two novel immunomodulatory proteins, MFAP5 and PENK that when administered to mice subjected to endotoxemic shock, reversed the cytokine storm and provided a significant survival benefit PMID: 24496384
    9. Decreased MFAP5 gene expression in the endometrium of patients with implantation failure after in vitro ertilization treatment PMID: 22885067
    10. The MAGP2-based assay provided superior performance for the purpose of cell culture identification compared to assays using standard reference genes. PMID: 20345228
    11. microfibrillar proteins MAGP-1 and MAGP-2 can function outside of their role in elastic fibers to activate a cellular signaling pathway PMID: 16492672
    12. MAGP-2 promotes angiogenic cell spouting in vitro by antagonizing Notch signaling pathways in endothelial cells. PMID: 18417156
    13. Independent evaluation confirmed the association of a prognostic gene microfibril-associated glycoprotein 2 (MAGP2) with poor prognosis in advanced ovarian cancer PMID: 19962670

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  • 相关疾病：
    Aortic aneurysm, familial thoracic 9 (AAT9)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    MFAP family
  • 数据库链接：

    HGNC: 29673

    OMIM: 601103

    KEGG: hsa:8076

    STRING: 9606.ENSP00000352455

    UniGene: Hs.512842

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