Human Methemoglobin,MHB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人高铁血红蛋白（MHB）酶联免疫检测试剂盒（货号：CSB-E09493h），采用双抗体夹心法原理进行定量分析，适用于检测血清、血浆样本中的MHB含量。高铁血红蛋白是血红蛋白的氧化形式，在缺氧、遗传性高铁血红蛋白血症或某些药物/化学物质暴露导致的溶血性疾病中可能异常升高，其水平变化可为氧化应激相关机制研究提供参考。该试剂盒检测范围为0.47-30 μg/mL，最低检测灵敏度达0.117 μg/mL，实验全程约需4小时，操作时仅需50 μL样本即可完成检测，配备96孔板规格满足高通量科研需求。本产品适用于基础医学研究中探索溶血性贫血、药物毒性反应、环境污染物暴露等场景下血红蛋白氧化损伤的动态变化，也可用于体外实验中评估化合物对红细胞氧化还原状态的影响，为氧化应激相关疾病机制研究及药物开发中的毒性评估提供定量检测工具。
缩写：

MHB
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.47 μg/mL-30 μg/mL
灵敏度：

0.11 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-91
1:200	Average %	87
	Range %	83-93
1:400	Average %	90
	Range %	87-93
1:800	Average %	95
	Range %	91-103

回收率：

The recovery of human MHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	88	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.