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  4. Human Melanoma-associated antigen D2(MAGED2) ELISA kit

Human Melanoma-associated antigen D2(MAGED2) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    人黑色素瘤相关抗原D2(MAGED2)酶联免疫试剂盒
  • 货号：
    CSB-EL013352HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人黑色素瘤相关抗原D2(MAGED2)酶联免疫试剂盒（CSB-EL013352HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MAGED2含量。MAGED2基因是MAGE家族成员之一，位于X染色体上，与X连锁智力障碍和某些癌症相关。其蛋白在细胞周期调节中发挥作用，可变剪接导致多个转录本变体。研究发现，MAGED2蛋白具有抗病毒功能，新冠病毒主蛋白酶可切割MAGED2蛋白，使其失去抗病毒作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品可广泛应用于肿瘤分子机制研究、抗癌药物体外筛选及肿瘤相关生物标志物的表达水平分析等科研领域，为探索MAGED2在肿瘤微环境调控、转移潜能评估等方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit
  • 缩写：
    MAGED2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 83-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 93
    Range % 88-97
    1:8 Average % 107
    Range % 102-111
  • 回收率：
    The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 102-108
    EDTA plasma (n=4) 96 91-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.238 2.334 2.286 2.090
    1000 1.415 1.527 1.471 1.275
    500 0.881 0.932 0.907 0.711
    250 0.560 0.573 0.567 0.371
    125 0.379 0.381 0.380 0.184
    62.5 0.292 0.284 0.288 0.092
    31.25 0.247 0.252 0.250 0.054
    0 0.195 0.196 0.196 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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相关产品

MAGED2 antibodies

MAGED2 Proteins

靶点详情

  • 功能：
    Regulates the expression, localization to the plasma membrane and function of the sodium chloride cotransporters SLC12A1 and SLC12A3, two key components of salt reabsorption in the distal renal tubule.
  • 基因功能参考文献：
    1. Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer. PMID: 25743330
    2. MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation. PMID: 26705694
    3. We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome. PMID: 27120771
    4. these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis PMID: 22791814
    5. Expression pattern and further characterization of human MAGED2 and identification of rodent orthologues PMID: 11856887
    6. Maged2 is mainly expressed in tissues of mesodermal origin PMID: 15162511
    7. Results identified a cDNA clone, corresponding to MAGE D2 mRNA, from primary human bronchial epithelial cells which exhibits increased expression in vitro after treatment with all-trans retinoic acid. PMID: 15465002
    8. MAGED2, a novel protein, is a p53-dissociator. PMID: 17912449

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  • 相关疾病：
    Bartter syndrome 5, antenatal, transient (BARTS5)
  • 组织特异性：
    Widely expressed. In the developing and adult kidney, expressed in the thick ascending limb of the loop of Henle and the distal convoluted tubules outside the loop.
  • 数据库链接：

    HGNC: 16353

    OMIM: 300470

    KEGG: hsa:10916

    STRING: 9606.ENSP00000218439

    UniGene: Hs.522665

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |