Human Mast Cell Carboxypeptidase ELISA Kit

Human Mast Cell Carboxypeptidase ELISA Kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人肥大细胞类羧肽酶酶联免疫试剂盒
货号：

CSB-E13756h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

人肥大细胞类羧肽酶酶联免疫试剂盒（CSB-E13756h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPA3含量。CPA3是一个值得关注的靶点。它在人体生理与病理过程中发挥作用。目前研究聚焦于其参与的免疫反应、炎症调节等机制。了解其作用机制有助于深入认识相关疾病发病原理，为开发针对性治疗手段如药物研发等提供重要方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对肥大细胞相关疾病机制的研究，如过敏性疾病模型构建、炎症标志物分析以及药物干预下CPA3表达水平变化的评估，为探索肥大细胞功能调控提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
缩写：

CPA3
Uniprot No.：

P15088
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	94-103
1:4	Average %	97
	Range %	94-100
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	94-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.477	2.453	2.465	2.366
10	1.993	1.924	1.959	1.860
5	1.280	1.271	1.276	1.177
2.5	0.685	0.678	0.682	0.583
1.25	0.433	0.424	0.429	0.330
0.625	0.286	0.269	0.278	0.179
0.312	0.190	0.179	0.185	0.086
0	0.101	0.097	0.099

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

基因功能参考文献：
1. Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID: 27157394
2. Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
3. The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID: 14760754
4. Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID: 19643669
亚细胞定位：

Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules.
蛋白家族：

Peptidase M14 family
数据库链接：

HGNC: 2298

OMIM: 114851

KEGG: hsa:1359

STRING: 9606.ENSP00000296046

UniGene: Hs.646

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.