Human Loricrin(LOR) ELISA kit

Human Loricrin(LOR) ELISA kit

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中文名称：

人兜甲蛋白(LOR)酶联免疫试剂盒
货号：

CSB-EL013037HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人兜甲蛋白（LOR）酶联免疫吸附检测试剂盒（货号：CSB-EL013037HU），基于双抗体夹心ELISA技术实现LOR的精准定量分析。LOR是表皮分化标志性蛋白，属于S100钙结合蛋白家族成员，参与皮肤屏障形成及角质化过程，其表达异常与银屑病、特应性皮炎等皮肤疾病相关。本试剂盒检测范围覆盖25 pg/mL至1600 pg/mL，可兼容血清、血浆、组织匀浆及细胞裂解液等多种样本类型，适用于科研场景中皮肤生物学机制研究、疾病模型表皮分化评估或药物干预效果分析。该检测体系通过预包被特异性捕获抗体与标记检测抗体的双重识别，确保高灵敏度和特异性，操作流程标准化且重复性良好。科研人员可通过本产品定量检测不同实验条件下LOR的表达水平变化，为探索皮肤屏障功能调控、表皮异常分化机制及相关疾病发病机理提供可靠工具。
别名：

LOR ELISA Kit; LOR protein ELISA Kit; LORI_HUMAN ELISA Kit; Loricrin ELISA Kit; LRN ELISA Kit; MGC111513 ELISA Kit; OTTHUMP00000015823 ELISA Kit
缩写：

LOR
Uniprot No.：

P23490
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	97
	Range %	94-100
1:4	Average %	98
	Range %	95-101
1:8	Average %	87
	Range %	82-91

回收率：

The recovery of human LOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	102	98-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.424	2.468	2.446	2.300
800	1.561	1.598	1.580	1.434
400	0.986	0.946	0.966	0.820
200	0.634	0.657	0.646	0.500
100	0.411	0.435	0.423	0.277
50	0.295	0.275	0.285	0.139
25	0.202	0.213	0.208	0.062
0	0.145	0.147	0.146

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

A lipid mixture enriched by ceramide NP with fatty acids of diverse chain lengths contributes to restore the skin barrier function impaired by topical corticosteroid SH Lim,Skin pharmacology and physiology,2023

功能：

Major keratinocyte cell envelope protein.
基因功能参考文献：
1. Authors report a multi-generation family with prominent ichthyosis and palmoplantar involvement due to a novel mutation in loricrin. PMID: 25965869
2. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
3. Studies on human keratinocytes recognized that loricrin expression was inversely related to the expression of the cyclin-dependent kinase inhibitor p21 PMID: 25896246
4. two novel heterozygous frameshift mutations in exon 2 - c.646_647insGCAGCAGGTC, p.Gln216Argfs*123 and c.798_799dupT, p.Gly267Trpfs*69 in loricrin keratoderma patients PMID: 25234742
5. Results describe a novel frameshift mutation leading to loricrin keratoderma presenting with colloidion membrane PMID: 25142840
6. We found no mutations of Loricrin in two Progressive symmetrical erythrokeratoderma families. PMID: 23678955
7. We describe a young man who was a collodion baby and had the typical presentation of Loricrin keratoderma. Direct DNA sequencing identified a heterozygous mutation in the loricrin gene with a single G insertion, 730insG, present in (a) the patient PMID: 22831754
8. There were no mutations found in the LOR gene and the true pathogenesis of progressive symmetrical erythrokeratodermia remains unknown. PMID: 21198793
9. These findings suggest that inverse effects of PKCdelta and PKCeta on loricrin expression attributes to the expression of c-Jun and JunD. PMID: 20184865
10. regulation of gene expression requires interactions among multiple transcription factors in keratinocytes located in different compartments of the epidermis PMID: 12200429
11. Unique mutations in glycine-rich domain of mutant loricrin form arginine-rich nuclear localization sequences that disrupt differentiation of keratinocytes. PMID: 12615358
12. Barrier abnormality in loricrin keratoderma is linked to defective CE scaffold, resulting in increased extracellular permeability. PMID: 15102081
13. ruled out as a candidate for the PSORS4 locus. PMID: 15598222
14. report the clinical and molecular characterization of a new family with the recurrent 730insG LOR mutation, giving new insights in LK genotype-phenotype correlation PMID: 17953701
15. results give evidence that heterogeneous phenotypes of LK may be the result of genetic heterogeneity of loricrin mutations, and demonstrate that nuclear accumulation of mutant loricrin is due to the nuclear targeting sequences in the mutant C-terminus. PMID: 18844868
16. the deregulated increase in SPRR1A expression in chronic atopic skin lesions reflects an insufficient rise in SPRR transcripts, unable to compensate for the lack of LOR and thus contributing to the persistence of chronic atopic dermatitis skin lesions. PMID: 19672094
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相关疾病：

Vohwinkel syndrome with ichthyosis (VSI)
亚细胞定位：

Cytoplasm. Nucleus, nucleoplasm.
数据库链接：

HGNC: 6663

OMIM: 152445

KEGG: hsa:4014

STRING: 9606.ENSP00000357731

UniGene: Hs.251680

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.