Human Long palate, lung and nasal epithelium carcinoma-associated protein 4(C20orf186) ELISA kit

Human Long palate, lung and nasal epithelium carcinoma-associated protein 4(C20orf186) ELISA kit

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中文名称：

人腭/肺/鼻上皮癌关联蛋白4(C20orf186)酶联免疫试剂盒
货号：

CSB-EL003694HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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本试剂盒用于定量检测人腭/肺/鼻上皮癌关联蛋白4（C20orf186）的浓度，该蛋白定位于20号染色体开放阅读框，在多种上皮组织癌变过程中可能参与细胞增殖或分化调控，是癌症机制研究的重要靶标。本产品采用双抗体夹心酶联免疫吸附法（ELISA），检测灵敏度为25 pg/mL，线性范围覆盖25-1600 pg/mL，适用于血清、血浆及组织匀浆样本的定量分析。试剂盒包含预包被特异性抗体的微孔板、标准品及检测所需全套试剂，通过生物素标记的检测抗体与链霉亲和素-HRP系统实现高灵敏度信号放大，操作流程标准化且重复性良好。该产品可应用于基础科研领域，如探究C20orf186在鼻咽癌、肺癌等上皮源性肿瘤中的表达特征，评估其作为潜在生物标志物的相关性，或用于药物干预实验中靶蛋白水平变化的动态监测，为肿瘤发生机制研究及治疗策略开发提供实验支持。
别名：

BPI fold-containing family B member 4 ELISA Kit; Bpifb4 ELISA Kit; C20orf186 ELISA Kit; dJ726C3.5 ELISA Kit; Gm1006 ELISA Kit; Ligand-binding protein RY2G5 ELISA Kit; Long palate; lung and nasal epithelium carcinoma-associated protein 4 ELISA Kit; LPLC4_HUMAN ELISA Kit; Lplunc4 ELISA Kit
缩写：

C20orf186
Uniprot No.：

P59827
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	98
	Range %	92-106
1:4	Average %	84
	Range %	81-89
1:8	Average %	91
	Range %	83-98

回收率：

The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.908	3.158	3.033	2.897
800	2.304	2.433	2.369	2.233
400	1.478	1.475	1.477	1.341
200	0.686	0.707	0.697	0.561
100	0.497	0.503	0.500	0.364
50	0.263	0.277	0.270	0.134
25	0.230	0.244	0.237	0.101
0	0.140	0.132	0.136

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Plasma Levels of Bactericidal/Permeability-Increasing Protein Correlate with Systemic Inflammation in Acute Coronary Syndrome S Yu,Research square,2021
The longevity-associated variant of BPIFB4 improves a CXCR4-mediated striatum–microglia crosstalk preventing disease progression in a mouse model of Huntington’s disease A Di Pardo,Cell Death & Disease,2020
Serum BPIFB4 levels classify health status in long-living individuals Villa F. et al,Immun Ageing,2015

功能：

May have the capacity to recognize and bind specific classes of odorants. May act as a carrier molecule, transporting odorants across the mucus layer to access receptor sites. May serve as a primary defense mechanism by recognizing and removing potentially harmful odorants or pathogenic microorganisms from the mucosa or clearing excess odorant from mucus to enable new odorant stimuli to be received.
基因功能参考文献：
1. LAV-BPIFB4 isoform modulates eNOS signalling through Ca2+/PKC-alpha-dependent mechanism. PMID: 28419216
2. In conclusion, BPIFB4 and CXCR4 expression classify long-lived individuals health status that correlates with maintained mononuclear cell migration. PMID: 28121621
3. Longevity-associated variant-BPIFB4 may represent a novel therapeutic tool to fight endothelial dysfunction and promote vascular reparative processes. PMID: 26034043
亚细胞定位：

Secreted. Cytoplasm.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
组织特异性：

Expressed in nasal tissue.
数据库链接：

HGNC: 16179

OMIM: 615718

KEGG: hsa:149954

STRING: 9606.ENSP00000364632

UniGene: Hs.38961

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.