Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit
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中文名称:
人肝表达抗菌肽2(LEAP2)酶联免疫试剂盒
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货号:
CSB-EL012853HU
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规格:
96T/48T
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价格:
¥3600/¥2500
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其他:
产品详情
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产品描述:
人肝表达抗菌肽2(LEAP2)酶联免疫试剂盒(CSB-EL012853HU)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LEAP2含量。人肝表达抗菌肽2(LEAP2)是一种新型活性多肽,具有抑菌和调节体重功能。其结构稳定,在先天免疫系统中发挥关键作用,能抵御细菌感染,并参与调节食欲、能量代谢等生理过程。LEAP2通过破坏细菌细胞膜和调节免疫细胞功能来发挥其抗菌和免疫调节作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL,支持科研人员探索LEAP2在代谢性疾病模型中的动态变化、评估肝脏功能状态,或研究其与微生物感染相关的免疫调节机制。为肝脏代谢研究、免疫调控机制探索及药物开发提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:
LEAP2Liver-expressed antimicrobial peptide 2 ELISA Kit; LEAP-2 ELISA Kit
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缩写:
LEAP2
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Uniprot No.:
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种属:
Homo sapiens (Human)
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样本类型:
serum, plasma, tissue homogenates
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检测范围:
0.312 ng/mL-20 ng/mL
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灵敏度:
0.078 ng/mL
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反应时间:
1-5h
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样本体积:
50-100ul
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检测波长:
450 nm
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研究领域:
Cardiovascular
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测定原理:
quantitative
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测定方法:
Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LEAP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:1 | Average % | 105 | | |
Range % | 98-111 | | |
1:2 | Average % | 99 | | |
Range % | 90-104 | | |
1:4 | Average % | 97 | | |
Range % | 86-103 | | |
1:8 | Average % | 103 | | |
Range % | 95-107 | | |
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回收率:
The recovery of human LEAP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 99 | 91-104 | | |
EDTA plasma (n=4) | 102 | 95-107 | | |
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.512 | 2.600 | 2.556 | 2.403 | | | 10 | 2.101 | 1.978 | 2.040 | 1.887 | | | 5 | 1.403 | 1.415 | 1.409 | 1.256 | | | 2.5 | 0.824 | 0.844 | 0.834 | 0.681 | | | 1.25 | 0.489 | 0.501 | 0.495 | 0.342 | | | 0.625 | 0.311 | 0.323 | 0.317 | 0.164 | | | 0.312 | 0.231 | 0.226 | 0.229 | 0.076 | | | 0 | 0.156 | 0.150 | 0.153 | | | |
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本试剂盒所含材料:
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LEAP2 antibody.
- Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated LEAP2 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
- Substrate A (1 x 7 ml)
- Substrate B (1 x 7 ml)
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理:
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货期:
3-5 working days
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