Human Lipocalin-15(LCN15) ELISA kit

中文名称：

人脂质运载蛋白-15(LCN15)酶联免疫试剂盒
货号：

CSB-EL012813HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人脂质运载蛋白-15(LCN15)酶联免疫试剂盒（CSB-EL012813HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LCN15含量。LCN15（脂质运载蛋白15）位于人类第9号染色体，主要在十二指肠、结肠和其他组织中表达。其功能与转运活性和小分子结合相关，涉及维生素、核苷和相关分子的运输以及葡萄糖和其他糖、胆汁盐和有机酸、金属离子和胺化合物的运输。LCN15与某些疾病相关，如逆行性遗忘症。研究LCN15的机制有助于理解其在疾病发生和发展中的作用。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研中探索LCN15在脂质转运机制、代谢调控网络或相关疾病模型中的功能研究；科研人员可利用定量分析不同样本来源的LCN15含量，为代谢性疾病、肥胖相关研究或炎症机制探索提供可靠的数据支持，满足体外实验模型、动物研究及生物标志物筛选等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LCN15 ELISA Kit; UNQ2541/PRO6093 ELISA Kit; Lipocalin-15 ELISA Kit
缩写：

LCN15
Uniprot No.：

Q6UWW0
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.68 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LCN15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	90
	Range %	86-97
1:4	Average %	96
	Range %	90-102
1:8	Average %	94
	Range %	91-98

回收率：

The recovery of human LCN15 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.083	2.252	2.168	2.038
600	1.648	1.699	1.674	1.544
300	1.144	1.165	1.155	1.025
150	0.692	0.721	0.707	0.577
75	0.446	0.457	0.452	0.322
37.5	0.308	0.326	0.317	0.187
18.75	0.215	0.225	0.220	0.090
0	0.129	0.131	0.130

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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亚细胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
数据库链接：

HGNC: 33777

KEGG: hsa:389812

STRING: 9606.ENSP00000313833

UniGene: Hs.413902

Human Lipocalin-15(LCN15) ELISA kit

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