Human Leucine-rich repeat-containing protein 17(LRRC17) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人富亮氨酸重复序列蛋白17(LRRC17)酶联免疫试剂盒（CSB-EL013112HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LRRC17含量。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，该产品适用于基础科研领域，可用于探究LRRC17在肿瘤微环境、免疫调节或神经退行性疾病中的表达变化，为相关分子机制研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LRRC17 ELISA Kit; P37NB ELISA Kit; UNQ3076/PRO9909 ELISA Kit; Leucine-rich repeat-containing protein 17 ELISA Kit; p37NB ELISA Kit
缩写：

LRRC17
Uniprot No.：

Q8N6Y2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

23.44 pg/mL-1500 pg/mL
灵敏度：

5.86 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LRRC17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	92
	Range %	85-98
1:4	Average %	86
	Range %	80-92
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of human LRRC17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	95	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors.
基因功能参考文献：
1. low circulating plasma level of LRRc17 might serve as an independent and additive risk factor for osteoporotic fractures. PMID: 27355564
2. Whole-genome microarray mRNA-expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly differentially expressed in these two cell types: NKX2-3 and LRRC17 in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts PMID: 27036009
亚细胞定位：

Secreted, extracellular space.
组织特异性：

Expressed in osteoblast cell lines. Well expressed in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung and only at the basal levels in the other tissues examined including adult kidney. More expressed in S-type neuroblastoma cells
数据库链接：

HGNC: 16895

KEGG: hsa:10234

STRING: 9606.ENSP00000344242

UniGene: Hs.567412