Human L-Carnitine ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人左旋肉碱/L-肉碱（L-Carnitine）酶联免疫检测试剂盒（货号：CSB-E13606h），采用双抗体夹心法定量检测血清、血浆、尿液及组织匀浆样本中L-肉碱的含量。L-肉碱是人体内重要的脂肪酸代谢载体，在能量生成过程中负责将长链脂肪酸转运至线粒体进行β氧化，其水平变化与代谢性疾病、运动机能及营养状态密切相关。试剂盒检测范围为0.312-20 ng/mL，最低检测限低至0.156 ng/mL，可在3小时内完成样本分析，适用于代谢调控机制研究、运动医学评估、营养代谢实验及药物开发中的生物标志物检测。该试剂盒经严格验证，可兼容多种生物样本类型，操作流程标准化，配套提供预包被酶标板和完整试剂体系，满足实验室对脂肪酸代谢、心肌能量代谢及线粒体功能相关研究的精准定量需求。本产品仅限科学研究使用，为探索L-肉碱在能量代谢异常、肥胖症或运动恢复等领域的分子机制提供可靠工具。
缩写：

L-Carnitine
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	88
	Range %	80-96
1:400	Average %	97
	Range %	91-105
1:800	Average %	99
	Range %	92-110
1:1600	Average %	100
	Range %	90-110

回收率：

The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-110
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
20	2.749	2.639	2.694	2.543
10	2.010	1.976	1.993	1.842
5	1.303	1.294	1.299	1.148
2.5	0.889	0.839	0.864	0.713
1.25	0.561	0.524	0.543	0.392
0.625	0.371	0.359	0.365	0.214
0.312	0.256	0.239	0.248	0.097
0	0.155	0.147	0.151