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  4. Human Keratin, type II cytoskeletal 6A(KRT6A) ELISA kit

Human Keratin, type II cytoskeletal 6A(KRT6A) ELISA kit

说明书下载
  • 中文名称：
    人角蛋白II型细胞骨架6A(KRT6A)酶联免疫试剂盒
  • 货号：
    CSB-EL012561HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人角蛋白II型细胞骨架6A（KRT6A）酶联免疫检测试剂盒（货号：CSB-EL012561HU），采用双抗体夹心法定量检测血清、血浆、组织匀浆及细胞裂解液样本中的KRT6A蛋白水平。KRT6A属于角蛋白家族成员，是表皮细胞分化的重要标志物，参与维持细胞机械稳定性，其异常表达与皮肤病理性增生、肿瘤发生等研究密切相关。试剂盒检测灵敏度达0.156 ng/mL，线性范围为0.156-10 ng/mL，覆盖常见科研样本的检测需求，操作流程包含标准品预包被板、生物素标记检测抗体及HRP-链霉亲和素放大系统，支持96孔板规格的批量检测。适用于基础医学研究中KRT6A蛋白在皮肤疾病模型、肿瘤微环境分析、细胞应激反应等场景的动态监测，可配合基因编辑、药物干预等实验方案开展分子机制探索，为上皮细胞生物学研究提供可靠的定量分析工具。
  • 别名：
    CK-6A ELISA Kit; CK-6D ELISA Kit; CK6A ELISA Kit; CK6C ELISA Kit; CK6D ELISA Kit; Cytokeratin-6A ELISA Kit; Cytokeratin-6D ELISA Kit; K2C6A_HUMAN ELISA Kit; K6A ELISA Kit; K6C ELISA Kit; K6D ELISA Kit; keratin 6A ELISA Kit; Keratin ELISA Kit; Keratin; type II cytoskeletal 6A ELISA Kit; Keratin-6A ELISA Kit; Krt6a ELISA Kit; KRT6C ELISA Kit; KRT6D ELISA Kit; type II cytoskeletal 6A ELISA Kit; Type-II keratin Kb6 ELISA Kit
  • 缩写：
    KRT6A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KRT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-91
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 103
    Range % 98-108
    1:8 Average % 90
    Range % 85-94
  • 回收率：
    The recovery of human KRT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-103
    EDTA plasma (n=4) 94 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.098 2.102 2.100 1.955
    5 1.721 1.793 1.757 1.612
    2.5 1.382 1.421 1.402 1.257
    1.25 1.090 1.150 1.120 0.975
    0.625 0.695 0.714 0.705 0.560
    0.312 0.459 0.436 0.448 0.303
    0.156 0.267 0.262 0.265 0.120
    0 0.142 0.147 0.145 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

KRT6A antibodies

KRT6A Proteins

靶点详情

  • 功能：
    Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
  • 基因功能参考文献：
    1. Manipulating K6a phosphorylation or ubiquitin-proteasome system (UPS) activity may provide opportunities to harness the innate immunity of epithelia against infection. PMID: 29191848
    2. Several missense polymorphisms in KRT6A, KRT6B and KRT6C that lead to a higher risk for dental caries. PMID: 29357356
    3. KRT6A genetic mutation is associate with the development of Pachyonychia Congenita in patients in Australia. PMID: 27041546
    4. we here describe a family with pachyonychia congenita K6a, manifesting atypical symptoms of impaired wound healing and cheilitis. PMID: 24708461
    5. Keratin-derived antimicrobial peptides (KDAMPs) and their synthetic analogs exhibit antimicrobial activity against bacterial pathogens. PMID: 23006328
    6. Genotype-phenotype correlations among PC patients with codon-125 mutation in KRT16 were established, while the phenotypes caused by the IVS8-2A>C mutation in KRT6A need further studies to confirm the rare feature of fissured tongue PMID: 22668561
    7. We observed a higher likelihood of oral leukokeratosis in individuals harboring KRT6A mutations. PMID: 22264670
    8. Phenotypic differences exist between KRT6A and KRT16 mutations support adoption of a new classification system. PMID: 22098151
    9. This report is the first case of pachyonychia congenita with laryngeal obstruction in which the gene mutation has been established (a deletional mutation in keratin 6a). PMID: 21554383
    10. Focal palmoplantar keratoderma is associated with mutations in keratin K6c in 3 families. 2 unrelated families have Asn172 del and the other has a deletion of AA 462-470. Review. PMID: 20470930
    11. these data highlight the possibility of a physiological role for K6/K16 heterodimers in keratinocyte cell migration, in addition to the heterodimer's known functions in cell differentiation and mechanical resilience. PMID: 20403371
    12. The mutation of 521T--> C in the K6A gene is the causing mutation in pachyonychia congenita type I. PMID: 20140871
    13. Mutations Y465H and N171D of the KRT16A gene were detected in the sporadic pachyonychia congenita cases. PMID: 19806570
    14. Four new missense and five known mutations in K6a, one new deletion and three previously identified missense mutations in K16, plus one known mutation in K17 are reported in pachyonychia congenita. PMID: 17719747
    15. PC-1 is due to mutations of the KRT16 gene or its expression partner KRT6A, wheres PC-2 is caused by mutations in the KRT17 or KRT6B genes. PMID: 18489596
    16. Three novel and four recurrent keratin 6A (KRT6A) mutations were found in Chinese patients with pachyonychia congenita type 1 PMID: 19416275
    17. Rapamycin selectively inhibits expression of an inducible keratin (K6a) in human keratinocytes and improves symptoms in pachyonychia congenita patients. PMID: 19699613

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  • 相关疾病：
    Pachyonychia congenita 3 (PC3)
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the corneal epithelium (at protein level).
  • 数据库链接：

    HGNC: 6443

    OMIM: 148041

    KEGG: hsa:3853

    STRING: 9606.ENSP00000369317

    UniGene: Hs.700779

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