Human Interferon epsilon(IFNE) ELISA kit

Human Interferon epsilon(IFNE) ELISA kit

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中文名称：

人干扰素ε(IFNE)酶联免疫试剂盒
货号：

CSB-EL011049HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人干扰素ε(IFNE)酶联免疫试剂盒（CSB-EL011049HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNE含量。IFNE（干扰素-ε）是一种免疫调节蛋白，具有抗病毒和抗菌作用。其研究机制涉及调节免疫细胞功能，增强免疫应答。IFNE在生物医学中的应用包括病毒和细菌感染的治疗，以及免疫相关疾病的预防和治疗。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于科研领域对IFNE表达水平的定量分析，例如研究病毒感染模型中IFNE的动态变化、探索炎症性疾病中黏膜免疫调节机制，或评估生殖系统相关疾病中IFNE的潜在生物标志物功能，为免疫学、病毒学及生殖医学研究提供可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit
缩写：

IFNE
Uniprot No.：

Q86WN2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-95
1:2	Average %	93
	Range %	88-99
1:4	Average %	92
	Range %	87-100
1:8	Average %	95
	Range %	88-99

回收率：

The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	93	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.058	2.011	2.035	1.931
500	1.664	1.547	1.606	1.502
250	1.188	1.149	1.169	1.065
125	0.739	0.704	0.722	0.618
62.5	0.561	0.521	0.541	0.437
31.2	0.375	0.354	0.365	0.261
15.6	0.219	0.211	0.215	0.111
0	0.105	0.103	0.104

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Defining a Role for Interferon Epsilon in Normal and Complicated Pregnancies D Miller,Heliyon,2022

功能：

Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections.
基因功能参考文献：
1. IFN epsilon protects macrophages against HIV infection through a type I IFN independent mechanism. PMID: 27942584
2. human IFNvarepsilon suppresses HIV replication at multiple stages of infection. PMID: 28045025
3. female sex workers decreased susceptibility to HIV-1 infection also have increased levels of IFNE gene and protein expression in the cervical epithelium PMID: 26555708
4. T Allele of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-epsilon is a risk factor for the development of intracerebral hemorrhage. PMID: 24055696
5. Full-length IFN-epsilon 5'UTR markedly suppressed mRNA expression under basal conditions. It contains 2 stable stem-loop structures which associate with importin 9. Loop 1 is essential for regulation of mRNA expression. PMID: 23851686
6. genetic polymorphism is related to onset time of vitiligo in Korean patients PMID: 23802172
7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20574843
8. Observational study of gene-disease association. (HuGE Navigator) PMID: 20588308
9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20601674
10. The structue and mRNA expression pattern of IFN-epsilon1 suggest that it may have a function distinct from those other members of type I INF. PMID: 15233997
11. TNF-alpha leads to stabilization of IFN-epsilon mRNA, increased IFN-epsilon synthesis, engagement of type I IFNRs, increased STAT1 expression and phosphorylation, and up-regulation of retinoic acid-inducible gene-I expression PMID: 17878351
12. A meta-analysis and a single-nucleotide polymorphism (SNP) rs1333049 representing the 9p21.3 locus provide unprecedented evidence for association between genetic variants at chromosome 9p21.3 and risk of coronary artery disease. PMID: 18362232
13. In macrophages IFN-tau increased the synthesis of 2',5'-oligoadenylate synthetase/RNase L, MxA protein, IL-10 & IL-6, but not of IL-1ss or TNF-alpha. PMID: 18842358
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亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
组织特异性：

Endometrium-specific.
数据库链接：

HGNC: 18163

OMIM: 615223

KEGG: hsa:338376

STRING: 9606.ENSP00000418018

UniGene: Hs.682604

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.