Human Interferon alpha-21(IFNA21) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人干扰素α-21(IFNA21)酶联免疫试剂盒（CSB-EL011039HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNA21含量。人干扰素α-21（IFNA21）是一种具有抗病毒和抗增殖活性的蛋白质，通过结合细胞表面受体IFNAR1和IFNAR2，激活JAK-STAT信号通路，调节免疫反应。其研究涉及基因改组技术，旨在提高其抗病毒和抗增殖活性，用于治疗多种疾病。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于科研领域对感染性疾病、自身免疫反应或肿瘤微环境等研究中IFNA21表达水平的定量分析，可用于评估病毒感染模型、免疫调节实验或药物干预后生物样本中IFNA21的动态变化，为探索其生物学功能及作用机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IFNA21 ELISA Kit; Interferon alpha-21 ELISA Kit; IFN-alpha-21 ELISA Kit; Interferon alpha-F ELISA Kit; LeIF F ELISA Kit
缩写：

IFNA21
Uniprot No.：

P01568
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNA21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-101
1:2	Average %	104
	Range %	98-107
1:4	Average %	87
	Range %	83-91
1:8	Average %	94
	Range %	90-97

回收率：

The recovery of human IFNA21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-94
EDTA plasma (n=4)	101	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
数据库链接：

HGNC: 5424

OMIM: 147584

KEGG: hsa:3452

STRING: 9606.ENSP00000369574

UniGene: Hs.113211