Human Inter-alpha-trypsin inhibitor heavy chain H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)ELISA kit
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中文名称：人间-alpha-胰蛋白酶抑制剂重链H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)酶联免疫试剂盒
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货号：CSB-E17022h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO定量检测人血清、血浆及组织匀浆样本中α-胰蛋白酶抑制剂重链H4（ITIH4）的酶联免疫试剂盒，采用双抗体夹心法原理，具有高灵敏度和特异性。ITIH4是ITIH蛋白家族成员，参与细胞外基质稳定和炎症调控，在肝脏病理、肿瘤微环境调控及心血管疾病研究中显示出作为潜在生物标志物的价值。试剂盒线性检测范围为0.625-40 ng/mL，最低检测限达0.156 ng/mL，适用于体外定量分析多种生物样本中ITIH4的表达水平。该试剂盒提供预包被特异性捕获抗体的96孔板、检测抗体及全套实验组分，操作流程标准化，支持科研人员探究ITIH4在疾病发生发展中的作用机制、药物干预效果评估或炎症相关通路研究，为分子生物学、病理学及药理学领域的基础研究提供可靠工具。
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别名：35 kDa inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; gp120 ELISA Kit; H4P ELISA Kit; IHRP ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; ITIH4 ELISA Kit; ITIH4_HUMAN ELISA Kit; ITIHL1 ELISA Kit; OTTHUMP00000197120 ELISA Kit; OTTHUMP00000197121 ELISA Kit; OTTHUMP00000213834 ELISA Kit; OTTHUMP00000213869 ELISA Kit; PK-120 ELISA Kit; PK120 ELISA Kit; Plasma kallikrein sensitive glycoprotein 120 ELISA Kit; PRO1851 ELISA Kit
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缩写：ITIH4
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：0.625 ng/mL-40 ng/mL
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灵敏度：0.156 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-97 1:2 Average % 97 Range % 92-102 1:4 Average % 96 Range % 90-104 1:8 Average % 97 Range % 92-102 -
回收率：
The recovery of human ITIH4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 106 98-110 EDTA plasma (n=4) 95 91-105 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 40 2.539 2.438 2.489 2.329 20 2.047 1.946 1.997 1.837 10 1.312 1.301 1.307 1.147 5 0.755 0.766 0.761 0.601 2.5 0.463 0.452 0.458 0.298 1.25 0.337 0.326 0.332 0.172 0.625 0.242 0.245 0.244 0.084 0 0.160 0.159 0.160 -
数据处理：
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货期：3-5 working days
引用文献
- Usability of serum inter-α-trypsin inhibitor heavy chain 4 as a biomarker for assessing severity and predicting functional outcome after human aneurysmal subarachnoid hemorrhage: A prospective observational cohort study at a single institution H Tian,Clinica chimica acta; international journal of clinical chemistry,2023
- Proteomics changes after negative pressure wound therapy in diabetic foot ulcers Z Jia,Molecular medicine reports,2021
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靶点详情
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功能：Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
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基因功能参考文献：
- Low ITIH4 expression is associated with Hepatocellular Carcinoma. PMID: 28828637
- this report has further supported for associations of genetic variants in the ITIH4 and CALN1 genes with schizophrenia and provided the first evidence that the variants regulate ITIH4 AND CALN1 expression in the dorsolateral prefrontal cortex PMID: 26991396
- ITIH4 peptide isoform as a preterm birth biomarker and its associated SNP implications PMID: 26408095
- Serum ITIH4 may be a PM10-specific biomarker in COPD and may be related to inflammation. PMID: 25977605
- Four novel body mass index-associated loci near the KCNQ1(rs2237892), ALDH2/MYL2 (rs671, rs12229654), ITIH4 (rs2535633) and NT5C2 (rs11191580) genes are identified in East Asian-ancestry populations. PMID: 24861553
- Worse survival among HCC patients with low ITIH4. PMID: 24836184
- Isoform-specific ITIH4 glycosylation and utilization of O-glycosylation sites on ITIH4 differs between cell lines and serum. PMID: 24884609
- Expression of the 85 kDa ITIH4 was substantial in amyotrophic lateral sclerosis compared with controls or patients with muscular dystrophy, Alzheimer diseases, or Parkinson diseases PMID: 23436019
- A truncated fragment of inter-alpha-trypsin inhibitor heavy chain 4 was the sole protein found to be significantly enhanced in the prostate cancer patients compared to the controls. PMID: 23417432
- Findings suggest that ITI-H4 expression may be used as a biomarker, which could facilitate the development of novel diagnostic and therapeutic tools. PMID: 21331437
- Genetic variation at ITIH4 locus is one of the likely candidate determinants for plasma cholesterol metabolisms PMID: 14661079
- ITIH4 and MTJ1 co-immunoprecipitate from total liver protein extracts and SANT domain of HTJ1 protects the ITIH4(588-930) recombinant fragment PMID: 16271702
- The inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) protein was significantly present more in interstial cystitis than controls PMID: 18455532
- ITIH4 is an anti-inflammatory protein, and suggests that further investigation into its potential use in the diagnosis and prognosis of acute ischemic stroke is warranted. PMID: 19263524
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亚细胞定位：Secreted.
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蛋白家族：ITIH family
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组织特异性：Liver specific.
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数据库链接：
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