Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

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中文名称：

人Igκ链V-IV区(IGKV4-1)酶联免疫试剂盒
货号：

CSB-EL011443HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人Igκ链V-IV区(IGKV4-1)酶联免疫试剂盒（CSB-EL011443HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物.样本中的IGKV4-1含量。IGKV4 - 1 是重要的靶点。它在相关疾病的发生发展中可能发挥关键作用。研究机制方面，可能涉及到与免疫反应、细胞信号传导等过程的关联，通过对其深入研究，有望揭示疾病的发病机制，为开发针对性的治疗方案奠定基础。试剂盒检测范围为0.156 ng/ml-10 ng/ml，适用于科研领域中对B细胞功能、抗体生成机制及相关疾病模型的探索，例如免疫失调研究、抗体工程开发或肿瘤微环境分析等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IGKV4-1Immunoglobulin kappa variable 4-1 ELISA Kit; Ig kappa chain V-IV region B17 ELISA Kit; Ig kappa chain V-IV region JI ELISA Kit; Ig kappa chain V-IV region Len ELISA Kit; Ig kappa chain V-IV region STH ELISA Kit
缩写：

IGKV4-1
Uniprot No.：

P06312
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates.
检测范围：

0.156 ng/ml -10 ng/ml
灵敏度：

0.039 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGKV4-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	96
	Range %	89-103
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	86-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.069

2.034

2.051

1.922

1.347

1.367

1.357

1.228

2.5

0.797

0.828

0.812

0.683

1.25

0.428

0.409

0.419

0.289

0.625

0.294

0.311

0.302

0.173

0.312

0.235

0.243

0.239

0.110

0.156

0.157

0.164

0.160

0.031

0.134

0.125

0.130

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

V segment of the variable domain of immunoglobulins light chain that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
亚细胞定位：

Secreted. Cell membrane.
数据库链接：

HGNC: 5834

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.