Human Ig gamma-3 chain C region(IGHG3) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人免疫球蛋白G3亚型重链恒定区（IGHG3）是IgG抗体家族的重要成员，其独特的铰链区结构赋予IgG3较短的半衰期和更强的补体激活能力，在自身免疫性疾病（如系统性红斑狼疮）及感染免疫应答中发挥关键作用。本试剂盒（CSB-EL011158HU）采用双抗体夹心技术，可精准定量检测血清或血浆样本中的IGHG3蛋白，检测灵敏度达2.7 ng/mL，线性范围覆盖2000 ng/mL，操作流程包含预包被特异性抗体、样本孵育、HRP标记抗体结合及显色反应等标准化步骤，可在4小时内完成实验。该产品适用于科研领域中对IgG3亚型表达水平的动态监测，如探索自身免疫疾病病理机制、评估疫苗免疫效果、研究免疫调节药物干预后的体液免疫变化等，为免疫学研究和生物标志物发现提供可靠工具，所有实验需严格遵循生物安全规范及实验室标准操作流程。
别名：

IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
缩写：

IGHG3
Uniprot No.：

P01860
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

2.7 ng/mL-2000 ng/mL
灵敏度：

2.7 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	93-106
1:2000	Average %	90
	Range %	85-95
1:4000	Average %	87
	Range %	82-91
1:8000	Average %	93
	Range %	86-98

回收率：

The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
亚细胞定位：

Secreted. Cell membrane.
数据库链接：

HGNC: 5527

OMIM: 147120

UniGene: Hs.510635