Human Ig alpha-1 chain C region(IGHA1) ELISA kit

产品详情

产品描述：

人Ig α1链C区(IGHA1)酶联免疫试剂盒（CSB-EL011111HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGHA1含量。IGHA1作为靶点，在相关研究中有重要价值。它可能与特定生理病理机制有关，研究主要聚焦于其在疾病发生发展里的作用机制，探索以它为靶点开发针对性药物或治疗手段，有望为相关疾病的防治带来新突破。试剂盒检测范围为0.49 ng/mL-2000 ng/mL，适用于免疫学基础研究、黏膜相关疾病机制探索（如感染性疾病或过敏模型）、免疫缺陷评估等科研领域。实验样本兼容常规生物液体样本类型，尤其适合评估体液免疫应答动态或特定病理条件下IgA抗体的表达特征，为研究黏膜免疫调控、宿主 - 病原体互作等课题提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IGHA1 ELISA Kit; Immunoglobulin heavy constant alpha 1 ELISA Kit; Ig alpha-1 chain C region ELISA Kit; Ig alpha-1 chain C region BUR ELISA Kit; Ig alpha-1 chain C region TRO ELISA Kit
缩写：

IGHA1
Uniprot No.：

P01876
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.49 ng/mL-2000 ng/mL
灵敏度：

0.49 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGHA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	92
	Range %	85-103
1:10000	Average %	91
	Range %	85-99
1:20000	Average %	97
	Range %	90-108
1:40000	Average %	92
	Range %	83-100

回收率：

The recovery of human IGHA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-101
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. Ig alpha is the major immunoglobulin class in body secretions.
相关疾病：

A chromosomal aberration involving IGHA1 is found in multiple myeloma (MM) cell lines. Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein.
亚细胞定位：

Secreted. Cell membrane.
数据库链接：

HGNC: 5478

OMIM: 146900

UniGene: Hs.699841