Human ICOS ligand(ICOSLG) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人ICOS配体（ICOSLG）酶联免疫试剂盒（货号：CSB-EL010958HU）是一款用于定量检测血清、血浆及组织匀浆样本中ICOSLG蛋白含量的科研工具。ICOSLG作为免疫共刺激分子，通过与T细胞表面的ICOS受体结合，参与调控免疫细胞活化、分化及免疫耐受等重要生理过程，在自身免疫性疾病、肿瘤微环境等领域具有重要研究价值。本试剂盒基于双抗体夹心法原理，通过特异性捕获和检测抗体实现目标蛋白的高灵敏度定量分析，检测范围为93.75 pg/mL至6000 pg/mL，可精准覆盖低丰度样本的检测需求。该产品适用于免疫调节机制研究、肿瘤免疫治疗探索以及生物标志物分析等科研场景，操作流程标准化，支持多样本类型的高通量检测，为探索ICOSLG在免疫系统中的功能及调控网络提供可靠工具。
别名：

ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275
缩写：

ICOSLG
Uniprot No.：

O75144
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 pg/mL-6000 pg/mL
灵敏度：

23.4 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-94
1:2	Average %	96
	Range %	89-102
1:4	Average %	98
	Range %	95-106
1:5	Average %	95
	Range %	91-100

回收率：

The recovery of human ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-103
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
亚细胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, BTN/MOG family
组织特异性：

Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leuko
数据库链接：

HGNC: 17087

OMIM: 605717

KEGG: hsa:102723996

STRING: 9606.ENSP00000384432

UniGene: Hs.14155