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  4. Human Hyaluronidase PH-20(SPAM1) ELISA kit

Human Hyaluronidase PH-20(SPAM1) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    人透明质酸酶PH-20(SPAM1)酶联免疫试剂盒
  • 货号：
    CSB-EL022471HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人透明质酸酶PH-20（SPAM1）定量检测试剂盒（货号：CSB-EL022471HU），采用双抗体夹心法原理，可精准检测血清、血浆、尿液及组织匀浆样本中的PH-20蛋白含量。PH-20属于透明质酸酶家族，通过降解细胞外基质中的透明质酸参与精子穿透卵丘、组织重塑及肿瘤微环境调控等重要生物学过程，其异常表达与生殖功能及肿瘤转移等研究领域密切相关。试剂盒具备15.6-1000 pg/mL的宽检测范围，灵敏度达15.6 pg/mL，标准曲线R²值≥0.99，预包被板孔提供即用型便利，实验流程仅需约3小时完成。该产品适用于基础科研中对PH-20的定量分析，可用于探究生殖生物学中的受精机制、肿瘤侵袭转移中的基质重塑作用，或评估疾病模型中透明质酸代谢相关通路的调控机制。实验配套提供标准品、检测抗体及显色系统，兼容常规酶标仪在450 nm波长下的读数需求，建议用户根据样本类型选择适当的前处理方案以保证检测准确性。
  • 别名：
    epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit
  • 缩写：
    SPAM1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 97  
    Range % 91-102  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 85  
    Range % 80-89  
  • 回收率：
    The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 104 100-108  
    EDTA plasma (n=4) 87 82-93  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.374 2.452 2.413 2.313  
    500 1.619 1.676 1.648 1.548  
    250 0.936 1.004 0.970 0.870  
    125 0.537 0.549 0.543 0.443  
    62.5 0.381 0.374 0.378 0.278  
    31.2 0.257 0.259 0.258 0.158  
    15.6 0.168 0.164 0.166 0.066  
    0 0.102 0.098 0.100    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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相关问答

■ 常见问题解答
Q:

We wonder whether this kit could be used in detection of recommbinant
Hyaluronidase by CHO.
We look forward to your reply.

A:
Thanks for your inquiry!
CSB-EL022471HU Human Hyaluronidase PH-20 (SPAM1) ELISA kit
This kit is designed according to: https://www.uniprot.org/uniprot/P38567.
It detects Hyaluronidase PH-20 rather than Hyaluronidase. If you want to detect recommbinant Hyaluronidase PH-20 by CHO, sorry that we are not sure 100% if it can be detected out or not. The recombinant protein used in the kit is 293 cell derived. As it involves different expression systems and modifications after expression, we can not guarantee that it can be detected out. We suggest you do a pretest first.
Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 功能：
    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • 基因功能参考文献：
    1. Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
    2. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
    3. HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
    4. The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
    5. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
    6. SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Glycosyl hydrolase 56 family
  • 组织特异性：
    Testis.
  • 数据库链接：

    HGNC: 11217

    OMIM: 600930

    KEGG: hsa:6677

    STRING: 9606.ENSP00000345849

    UniGene: Hs.121494

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