-
中文名称:人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶联免疫试剂盒
-
货号:CSB-EL007812HU
-
规格:96T/48T
-
价格:¥3600/¥2500
-
其他:
产品详情
-
产品描述:CUSABIO试剂盒采用双抗体夹心法定量检测人血清、血浆或组织匀浆样本中的HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6),该蛋白由人类内源性逆转录病毒K家族(HERV-K)基因编码,其表达与多种生理调控及病理过程相关,在病毒与宿主相互作用、细胞分化及免疫调节等基础研究领域具有重要价值。试剂盒检测灵敏度为0.156 ng/mL,线性范围覆盖0.156-10 ng/mL,实验全程可在4.5小时内完成,适用于体外样本中ERVK6蛋白表达水平的精准分析,可为病毒进化研究、宿主基因组稳定性探索以及相关疾病模型的分子机制解析提供技术支持。配套提供预包被捕获抗体、生物素标记检测抗体及标准品体系,通过链霉亲和素-HRP系统显色,支持酶标仪在450nm波长下进行吸光度测定。该产品严格适用于科研场景,包括但不限于细胞实验、动物模型或生物样本库中ERVK6蛋白的动态监测,为分子生物学及病毒学领域的研究者提供稳定可靠的检测工具。
-
别名:ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
-
缩写:
-
Uniprot No.:
-
种属:Homo sapiens (Human)
-
样本类型:serum, plasma, tissue homogenates
-
检测范围:0.156 ng/mL-10 ng/mL
-
灵敏度:0.039 ng/mL
-
反应时间:1-5h
-
样本体积:50-100ul
-
检测波长:450 nm
-
研究领域:Others
-
测定原理:quantitative
-
测定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 91-99 1:2 Average % 103 Range % 98-106 1:4 Average % 110 Range % 106-114 1:8 Average % 92 Range % 89-97 -
回收率:
The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 81-89 EDTA plasma (n=4) 97 93-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.251 2.301 2.276 2.154 5 1.375 1.368 1.372 1.250 2.5 0.782 0.778 0.780 0.658 1.25 0.503 0.511 0.507 0.385 0.625 0.325 0.336 0.331 0.209 0.312 0.247 0.268 0.258 0.136 0.156 0.192 0.186 0.189 0.067 0 0.121 0.123 0.122 -
数据处理:
-
货期:3-5 working days
引用文献
- Corrigendum: Subtype-specific human endogenous retrovirus K102 envelope protein is a novel serum immunosuppressive biomarker of cancer Q Gong, R Xu,Frontiers in Immunology,2025
- Subtype-specific HERV-K102 envelope is a novel serum immunosuppressive biomarker and therapeutic target of cancer R Xu,/,2024
- Human endogenous retroviruses are upregulated in Frontotemporal Dementia-MAPT mutation carriers S Han,/,2024
- Nuclear lamina erosion-induced resurrection of endogenous retroviruses underlies neuronal aging H Zhang,Cell reports,2023
- Human endogenous retrovirus K (HERV-K) env in neuronal extracellular vesicles: a new biomarker of motor neuron disease Y Li,Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration,2021
相关产品
靶点详情
-
功能:Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
-
亚细胞定位:[Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.
-
蛋白家族:Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
-
组织特异性:Expressed in testis, and peripheral blood lymphocytes.
-
数据库链接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-