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  4. Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Human Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

说明书下载
  • 中文名称：
    人XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒
  • 货号：
    CSB-EL018086HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人XIIA组分泌型磷脂酶A2(PLA2G12A)酶联免疫试剂盒（CSB-EL018086HU）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLA2G12A含量。PLA2G12A是一种分泌型磷脂酶A2，具有低活性，主要在心脏、骨骼肌、肾脏、肝脏和胰腺中表达。其研究机制涉及磷脂代谢和类二十烷酸的产生，与多种生理和病理作用相关，如心血管疾病和神经发育障碍。试剂盒检测范围为4.7 pg/mL-300 pg/mL，该产品可应用于细胞脂质代谢研究、炎症相关信号通路探索、疾病动物模型生物标志物分析等科研场景，为探究PLA2G12A在病理生理过程中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    EC 3.1.1.4 ELISA Kit; Group XII secreted phospholipase A2 ELISA Kit; Group XIIA secreted phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; Group XIIA secretory phospholipase A2 precursor ELISA Kit; GXII ELISA Kit; GXII sPLA2 ELISA Kit; PG12A_HUMAN ELISA Kit; phosphatidylcholine 2 acylhydrolase 12A ELISA Kit; Phosphatidylcholine 2 acylhydrolase GXII ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit; Phospholipase A2 group XIIA ELISA Kit; PLA2G12 ELISA Kit; Pla2g12a ELISA Kit; ROSSY ELISA Kit; sPLA2 XII ELISA Kit; sPLA2-XII ELISA Kit
  • 缩写：
    PLA2G12A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.2 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:20 Average % 93    
    Range % 90-96    
    1:40 Average % 107    
    Range % 103-110    
    1:80 Average % 86    
    Range % 83-90    
    1:160 Average % 101    
    Range % 98-106    
  • 回收率：
    The recovery of human PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 85 80-90    
    EDTA plasma (n=4) 101 97-106    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average      
    300 0.236 0.230 0.233      
    150 0.386 0.391 0.389      
    75 0.589 0.592 0.591      
    37.5 0.847 0.834 0.841      
    18.75 1.076 1.100 1.088      
    9.4 1.534 1.502 1.518      
    4.7 1.952 1.991 1.972      
    0 2.345 2.401 2.373      
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

PLA2G12A antibodies

PLA2G12A Proteins

靶点详情

  • 功能：
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
  • 基因功能参考文献：
    1. The study demonstrated that PLA2G12A SNPs or haplotypes might influence the susceptibility to schizophrenia in the Han Chinese population from Northeast China. PMID: 27434078
    2. cellular arachidonate (AA) release and prostaglandin (PG) production are regulated by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII PMID: 12522102
  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Phospholipase A2 family
  • 组织特异性：
    Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
  • 数据库链接：

    HGNC: 18554

    OMIM: 611652

    KEGG: hsa:81579

    STRING: 9606.ENSP00000243501

    UniGene: Hs.389452

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |