Human Group IIF secretory phospholipase A2(PLA2G2F) ELISA kit

产品详情

产品描述：

人IIF组分泌型磷脂酶A2(PLA2G2F)酶联免疫试剂盒（CSB-EL018095HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、tissue,匀浆样本中的PLA2G2F含量。PLA2G2F，即磷脂酶A2家族IIF分泌型磷脂酶，是一种钙依赖性磷脂酶，主要靶向细胞外磷脂。其通过水解磷脂中的酯键释放脂肪酸，参与炎症条件下脂质介质的产生。PLA2G2F在炎症、免疫和代谢等多种生理过程中发挥重要作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于探索其在动脉粥样硬化、代谢紊乱等病理过程中的作用机制研究，或评估疾病模型中酶活性变化等科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

PLA2G2FGroup IIF secretory phospholipase A2 ELISA Kit; GIIF sPLA2 ELISA Kit; sPLA2-IIF ELISA Kit; EC 3.1.1.4 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 2F ELISA Kit
缩写：

PLA2G2F
Uniprot No.：

Q9BZM2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue,homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich
数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids (phospholipase A2 activity), the catalytic efficiency decreasing in the following order: phosphatidylglycerols > phosphatidylethanolamines > phosphatidylcholines > phosphatidylserines. May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases.
基因功能参考文献：
1. two of these isoforms, sPLA2 IIA and sPLA2 IIF, localize to the upper stratum granulosum and increase in response to experimental barrier perturbation. PMID: 24269828
亚细胞定位：

[Isoform 1]: Secreted. Cell membrane; Peripheral membrane protein.
蛋白家族：

Phospholipase A2 family
组织特异性：

Expressed at high levels in placenta, testis, thymus and at lower levels in heart, kidney, liver and prostate. Highly expressed in rheumatoid arthritic tissues, including synovial lining cells in the intima, capillary endothelial cells and plasma cells.
数据库链接：

HGNC: 30040

KEGG: hsa:64600

STRING: 9606.ENSP00000364243

UniGene: Hs.302034