Human Ganglioside GM2 activator(GM2A) ELISA kit

Human Ganglioside GM2 activator(GM2A) ELISA kit

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中文名称：

人神经节苷脂GM2活化剂(GM2A)酶联免疫试剂盒
货号：

CSB-EL009565HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人神经节苷脂GM2活化剂(GM2A)酶联免疫试剂盒（CSB-EL009565HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆、细胞裂解物样本中的GM2A含量。GM2A 是一个重要靶点，它与 GM2 神经节苷脂代谢相关。其研究机制主要围绕在辅助降解 GM2 神经节苷脂上。若 GM2A 功能异常，会导致 GM2 神经节苷脂蓄积，引发神经系统疾病，故围绕其开展的研究对相关疾病治疗意义重大。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于生命科学领域的基础研究，例如探究脂质代谢异常相关疾病的分子机制、评估疾病模型中GM2A的表达动态变化，或用于神经生物学、细胞生物学实验中分析特定干预条件对GM2A蛋白水平的影响，为研究该靶点在生理病理过程中的作用提供定量化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Cerebroside sulfate activator protein ELISA Kit; ganglioside GM2 activator ELISA Kit; Ganglioside GM2 activator isoform short ELISA Kit; Ganglioside GM2 activator precursor ELISA Kit; GM2 activator ELISA Kit; GM2 AP ELISA Kit; GM2 ganglioside activator ELISA Kit; GM2 ganglioside activator protein ELISA Kit; GM2-AP ELISA Kit; GM2A ELISA Kit; GM2AP ELISA Kit; OTTHUMP00000160619 ELISA Kit; SAP 3 ELISA Kit; SAP-3 ELISA Kit; SAP3 ELISA Kit; SAP3_HUMAN ELISA Kit; Shingolipid activator protein 3 ELISA Kit; Sphingolipid activator protein 3 ELISA Kit
缩写：

GM2A
Uniprot No.：

P17900
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GM2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	92
	Range %	87-99
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of human GM2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-103
EDTA plasma (n=4)	90	84-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.043	2.050	2.047	1.921
500	1.619	1.684	1.652	1.526
250	1.105	1.200	1.153	1.027
125	0.683	0.703	0.693	0.567
62.5	0.403	0.421	0.412	0.286
31.2	0.296	0.322	0.309	0.183
15.6	0.241	0.235	0.238	0.112
0	0.123	0.129	0.126

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

A Label-Free Electrochemical Biosensor for Highly Sensitive Detection of Gm2a Based on Gold Nanoparticles/Conducting Amino-Functionalized Thiophene Polymer Layer EB Aydin,SSRN,2023

功能：

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity.
基因功能参考文献：
1. Review of GM2A mutations causing GM2 activator protein deficiency and GM2 gangliosidosis-AB variant. PMID: 27402091
2. Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation. PMID: 27157270
3. this study has established the potential role of GM2A in breast cancer progression PMID: 27002480
4. Mobilization of membrane lipids by GM2AP was also inhibited in the presence of cholesterol or SM, as revealed by surface plasmon resonance studies PMID: 26175473
5. Gene polymorphisms of MD2 and GM2A were associated with the occurrence or severity of neonatal necrotizing enterocolitis. PMID: 25816011
6. In vitro assays with the isolated H1 or H2 homodimers (beta-alpha hybrid construct of beta-hexosaminidase A subunits) confirmed that neither was capable of human GM2AP-dependent hydrolysis of GM2 ganglioside. PMID: 23483939
7. impact of GM2AP on glucose metabolism PMID: 21784073
8. Treatment of meniscal explants with IL-1RA inhibited the expression of many catabolic genes following a single bout of high dynamic strain. PMID: 21331553
9. these results provide novel insights into the physiological functions of GM2AP in obesity. PMID: 21036149
10. GM2 activator protein exerts strong and broad inhibitory effects on the hydrolysis of phospholipids carried out by plant and microbial phospholipases D PMID: 12576516
11. Two new structures of GM2-AP with bound lipids, showing two different lipid-binding modes within the apolar pocket PMID: 12909021
12. elucidation of mode of action on gangioside GM2 PMID: 14728689
13. alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A PMID: 15485660
14. glycosphingolipids, particularly GM2, form a complex with CD82, and this complex interacts with Met and thereby inhibits HGF-induced Met tyrosine kinase activity, as well as integrin to Met cross-talk PMID: 17215249
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相关疾病：

GM2-gangliosidosis AB (GM2GAB)
亚细胞定位：

Lysosome.
数据库链接：

HGNC: 4367

OMIM: 272750

KEGG: hsa:2760

STRING: 9606.ENSP00000349687

UniGene: Hs.483873

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.