Human Galanin-like peptide(GALP) ELISA kit

Human Galanin-like peptide(GALP) ELISA kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人甘丙肽样肽(GALP)酶联免疫试剂盒
货号：

CSB-EL009222HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

CUSABIO人甘丙肽样肽（GALP）酶联免疫检测试剂盒（货号：CSB-EL009222HU），基于双抗体夹心法原理实现定量检测，适用于分析血清、血浆及组织匀浆样本中的GALP水平，检测灵敏度达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL。GALP作为甘丙肽家族成员，在中枢神经系统中参与调节摄食行为、能量代谢及神经内分泌功能，其表达异常与肥胖、糖尿病等代谢性疾病的研究密切相关。本试剂盒通过特异性抗体对实现精准捕获与信号放大，可稳定检测生物样本中GALP的浓度变化，为体外实验研究其生理病理机制提供可靠工具，尤其适用于探究代谢调控、神经内分泌网络及相关疾病模型的分子机制，满足基础科研中对GALP表达水平动态监测的需求。
别名：

GALP ELISA Kit; Galanin-like peptide ELISA Kit
缩写：

GALP
Uniprot No.：

Q9UBC7
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-97
1:2	Average %	101
	Range %	97-103
1:4	Average %	95
	Range %	88-98
1:8	Average %	99
	Range %	94-102

回收率：

The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-102
EDTA plasma (n=4)	94	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.057	2.080	2.069	1.918
5	1.761	1.812	1.787	1.636
2.5	1.403	1.373	1.388	1.237
1.25	0.988	0.973	0.981	0.830
0.625	0.703	0.727	0.715	0.564
0.312	0.477	0.497	0.487	0.336
0.156	0.322	0.331	0.327	0.176
0	0.150	0.152	0.151

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Alarin: A Novel Circulating Peptide Hormone Linked to Luteinizing Hormone and Hiperandrogenismin Polycystic Ovary Syndrome Merve Bicer, et al,Current opinion in obstetrics & gynecology,2018
Kisspeptin And Galanin-like Peptide (GALP) Levels In Women With Polycystic Ovary Syndrome PV Nyagolova.et al,/,/

功能：

Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2. Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing.
基因功能参考文献：
1. results indicated that obese individuals have higher plasma GAL and GALP concentrations and both peptide concentrations were positively correlative to triglyceride concentrations in obese human PMID: 27474259
2. One isoform (alarin) of this gene but not the other (galanin-like peptide) displays antimicrobial activity against E. coli. PMID: 23537644
3. This review presents considerable evidence that has accumulated implicating both galanin and GALP as playing important roles in regulating food and water intake behavior and related neuroendocrine functions. PMID: 16787232
4. describes cloning of the full-length human GALP PMID: 10601261
5. describes the gene structure of human GALP. PMID: 11861493
6. reports immunostaining of human GALP in the dermal microvasculature. PMID: 17535903
显示更多

收起更多
亚细胞定位：

Secreted.
蛋白家族：

Galanin family
组织特异性：

Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
数据库链接：

HGNC: 24840

OMIM: 611178

KEGG: hsa:85569

STRING: 9606.ENSP00000349884

UniGene: Hs.283915

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.