Human Fibulin-7(FBLN7) ELISA kit

产品详情

产品描述：

人腓骨蛋白-7(FBLN7)酶联免疫试剂盒（CSB-EL008455HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FBLN7含量。FBLN7，即纤连蛋白7，是一种胞外基质蛋白，参与多种蛋白间的相互作用。研究发现，FBLN7在心血管发育、牙齿发育、肿瘤转移等过程中发挥重要作用。其研究机制涉及调节细胞粘附、生长、迁移和存活等细胞功能。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研中FBLN7的蛋白表达分析，例如探究其在组织修复、胚胎发育或特定病理模型（如纤维化疾病、肿瘤微环境）中的动态变化，为体外实验或动物模型研究提供高效工具。通过兼容多种生物样本类型，研究者可灵活应用于体液检测或组织水平的分子机制探索，为细胞外基质相关研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

FBLN7 ELISA Kit; FBLN7_HUMAN ELISA Kit; FIBL-7 ELISA Kit; Fibl7 ELISA Kit; Fibulin-7 ELISA Kit; TM14 ELISA Kit
缩写：

FBLN7
Uniprot No.：

Q53RD9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-99
1:2	Average %	95
	Range %	87-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	97
	Range %	91-101

回收率：

The recovery of human FBLN7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	91	84-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

An adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
基因功能参考文献：
1. Fbln7-C terminal fragment inhibited the HUVEC tube formation and the vessel sprouting in aortic ring assays. PMID: 24480309
2. Dysregulation of fibulin expression by anti-Ro/SSA antibodiess may contribute to disorganization of the extracellular environment and thus cause injury to the salivary gland architecture and functionality observed in Sjogren syndrome PMID: 19229767
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibulin family
数据库链接：

HGNC: 26740

OMIM: 611551

KEGG: hsa:129804

STRING: 9606.ENSP00000331411

UniGene: Hs.437696