Human Fibroblast growth factor 5(FGF5) ELISA kit

Human Fibroblast growth factor 5(FGF5) ELISA kit

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中文名称：

人成纤维细胞生长因子5(FGF5)酶联免疫试剂盒
货号：

CSB-EL008632HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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人成纤维细胞生长因子5(FGF5)酶联免疫试剂盒（CSB-EL008632HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF5含量。FGF5（成纤维细胞生长因子5）是一种分泌信号蛋白，主要在间充质和骨骼肌中表达。它对哺乳动物毛发周期性生长起关键作用，同时与动物肢体发育及人类多形性胶质母细胞瘤、黑色素瘤、肝细胞癌和高血压有关。研究显示，FGF5通过调节细胞增殖和信号通路影响这些过程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外研究场景，如探究FGF5在毛囊生物学、皮肤病理模型或组织工程中的功能机制，亦可应用于药物开发中靶向分子效应的评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

FGF 5 ELISA Kit; FGF-5 ELISA Kit; FGF5 ELISA Kit; FGF5_HUMAN ELISA Kit; Fibroblast growth factor 5 ELISA Kit; HBGF 5 ELISA Kit; HBGF-5 ELISA Kit; heparin binding growth factor 5 ELISA Kit; Heparin-binding growth factor 5 ELISA Kit; Smag 82 ELISA Kit; Smag-82 ELISA Kit; TCMGLY ELISA Kit
缩写：

FGF5
Uniprot No.：

P12034
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	106
	Range %	100-113

回收率：

The recovery of human FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.314	2.227	2.271	2.126
500	1.816	1.876	1.846	1.701
250	1.334	1.402	1.368	1.223
125	0.801	0.840	0.821	0.676
62.5	0.544	0.518	0.531	0.386
31.2	0.372	0.359	0.366	0.221
15.6	0.271	0.282	0.277	0.132
0	0.142	0.147	0.145

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway F Chen,Journal of cellular and molecular medicine,2023

功能：

Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
基因功能参考文献：
1. Study indicated that MTHFR rs1801133, FGF5 rs16998073 and CSK rs1378942 were associated with increased the risk of obesity in the Chinese children. PMID: 30217759
2. FGF5 is an independent protective factor for breast cancer patients. PMID: 29804124
3. These results provide new insight into the functions of miR-9-3p and HBGF-5 in HCC and identify miR-9-3p as a potential therapeutic target for HCC PMID: 28750499
4. FGF5 facilitates cell proliferation through ERK1/2 activation, and it influences the osteogenic differentiation of tonsil-derived mesenchymal stem cells. PMID: 27224250
5. These findings collectively demonstrate a tumor suppressor role of miR-188-5p in HCC progression via targeting FGF5 PMID: 25998163
6. High BMI increases the effect of the blood pressure-increasing allele at rs1458038 near FGF5 in a Chinese population. PMID: 25618516
7. In Chinese children, no association of CSK rs1378942, MTHFR rs1801133, CYP17A1 rs1004467, STK39 rs3754777 and FGF5 rs16998073 with BP/risk of hypertension. PMID: 23759979
8. FGF5 is a crucial regulator of hair growth in humans. PMID: 24989505
9. Meta-analysis indicated significant associations of both CYP17A1 rs11191548 and FGF5 rs16998073 polymorphisms with hypertension susceptibility in East Asians. PMID: 22959498
10. Variants in or near FGF5, CYP17A1 and MTHFR contributed to variation in BP and hypertension risk. Effect sizes of these three loci tended to be larger in Chinese than in white Europeans PMID: 20852445
11. variation in FGF5 and ZNF652 gene upstream regions with altered susceptibility to hypertension in Han Chinese PMID: 20542020
12. The role of the proteasome is confirmed when a spliced FGF-5 peptide is produced in vitro after incubation of proteasomes with a 49-amino-acid precursor peptide in a transpeptidation splicing model. PMID: 20154207
13. FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects. PMID: 18362893
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相关疾病：

Trichomegaly (TCMGLY)
亚细胞定位：

Secreted.
蛋白家族：

Heparin-binding growth factors family
组织特异性：

Expressed in neonatal brain.
数据库链接：

HGNC: 3683

OMIM: 165190

KEGG: hsa:2250

STRING: 9606.ENSP00000311697

UniGene: Hs.37055

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.