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  4. Human Fetuin-B(FETUB) ELISA kit

Human Fetuin-B(FETUB) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    人胎球蛋白B(FETUB)酶联免疫试剂盒
  • 货号：
    CSB-EL008598HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人胎球蛋白B(FETUB)酶联免疫试剂盒（CSB-EL008598HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FETUB含量。试剂盒检测范围为0.137 ng/ml-100 ng/ml，灵敏度为0.137 ng/ml。适用于科研领域对血清、血浆及组织匀浆样本中FETUB蛋白含量的精准分析；为研究者探索FETUB在代谢调控、肝脏病理机制中的生物学功能提供可靠工具；用于相关疾病模型或干预实验中生物标志物的动态监测；适用于细胞实验、动物模型研究及人类样本分析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    16G2 ELISA Kit; 2310011O17Rik ELISA Kit; AI255764 ELISA Kit; D17980 ELISA Kit; Fet ELISA Kit; Fetub ELISA Kit; FETUB_HUMAN ELISA Kit; Fetuin beta ELISA Kit; Fetuin-B ELISA Kit; Fetuin-like protein ELISA Kit; Fetuin-like protein IRL685 ELISA Kit; Gugu ELISA Kit; IRL685 ELISA Kit; Pp63 ELISA Kit
  • 缩写：
    FETUB
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.137 ng/ml-100 ng/ml.
  • 灵敏度：
    0.137 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    12.720

    13.079

    12.874

    12.643

    12.823

    12.925

    SD

    0.023

    0.027

    0.025

    0.036

    0.033

    0.031

    CV(%)

    3.622

    4.160

    3.900

    5.696

    5.164

    4.821

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    106

    Range %

    102-108

    1:20

    Average %

    88

    Range %

    84-92

    1:40

    Average %

    92

    Range %

    85-96

    1:80

    Average %

    87

    Range %

    82-83

  • 回收率：

    The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    104

    98-108

    EDTA plasma (n=4)

    98

    94-102

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.775

    2.859

    2.817

    2.665

    33.33

    1.951

    1.937

    1.944

    1.792

    11.11

    1.090

    1.062

    1.076

    0.924

    3.7

    0.642

    0.691

    0.666

    0.514

    1.23

    0.385

    0.364

    0.375

    0.223

    0.41

    0.283

    0.285

    0.284

    0.132

    0.137

    0.218

    0.235

    0.226

    0.074

    0

    0.151

    0.153

    0.152

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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FETUB antibodies

FETUB Proteins

靶点详情

  • 功能：
    Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening.
  • 基因功能参考文献：
    1. High Serum fetuin-B expression is associated with insulin resistance. PMID: 29194974
    2. fetuin B may serve as a potential biomarker for coronary artery disease PMID: 28822077
    3. Increased serum fetuin-B level was significantly associated with higher risk of insulin resistance. Common genetic variants of FETUB SNPs (rs4686434, rs6785067, and rs3733159) were significantly associated with serum fetuin-B concentrations but were not associated with insulin resistance. PMID: 29390354
    4. serum level of fetuin B in the control subjects was lower than it in nonalcoholic fatty liver disease patients. fetuin B correlated with diastolic blood pressure, serum alanine transaminase and triglycerides, among the controls. It suggested a potential association between serum fetuin B and the presence of NAFLD. PMID: 27628583
    5. Further studies need to elucidate factors contributing to fetuin B regulation in humans, as well as the pathophysiological significance of fetuin B upregulation in gestational diabetes PMID: 28183456
    6. Data indicate that fetuin-B was one of the highly expressed serum proteins in acute myocardial infarction. PMID: 25671698
    7. Reduced expression of fetuin-b led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels. PMID: 22739111
    8. serum fetuin has a role in processing and transport of matrix gamma-carboxyglutamic acid protein and bone morphogenetic protein-2 in cultured human vascular smooth muscle cells PMID: 15280384
    9. results support the hypothesis that fetuin-A serum levels are associated with bone cell activity PMID: 16797392

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 组织特异性：
    Liver and testis.
  • 数据库链接：

    HGNC: 3658

    OMIM: 605954

    KEGG: hsa:26998

    STRING: 9606.ENSP00000265029

    UniGene: Hs.81073

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |