Human F-actin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人F-肌动蛋白（F-actin）定量检测的酶联免疫试剂盒（货号：CSB-E13678h），基于双抗体夹心法原理设计，适用于检测血清、血浆及组织匀浆样本中细胞骨架核心蛋白F-肌动蛋白的表达水平。F-肌动蛋白作为细胞形态维持、迁移及信号转导的关键调控因子，其动态聚合与解聚过程与细胞运动、胞质分裂等重要生物学功能密切相关。该试剂盒检测灵敏度高，线性范围覆盖0.625 ng/mL至40 ng/mL，实验全程可在4小时内完成，标准品采用梯度预包被设计，支持研究者通过酶标仪在450nm波长下快速获取定量数据。产品提供96孔板规格，包含完整实验所需预混试剂及操作说明书，适用于细胞骨架动态研究、细胞力学特性分析、心血管疾病或肿瘤转移机制探索等科研领域，尤其可为细胞应激反应、病理模型构建及药物干预效果评估提供可靠的分子检测工具。
缩写：

F-actin
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of human F-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-101
EDTA plasma (n=4)	88	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.