Human Enteropeptidase(PRSS7) ELISA kit

Human Enteropeptidase(PRSS7) ELISA kit

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中文名称：

人肠肽酶(PRSS7)酶联免疫试剂盒
货号：

CSB-EL018824HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人肠肽酶(PRSS7)酶联免疫试剂盒（CSB-EL018824HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRSS7含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于基础科研中探索PRSS7在胃肠功能调控、营养代谢相关实验模型中的表达动态，或用于评估特定干预措施对肠道蛋白酶活性的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

TMPRSS15; ENTK; PRSS7; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15
缩写：

PRSS7
Uniprot No.：

P98073
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-110
1:2	Average %	90
	Range %	85-85
1:4	Average %	92
	Range %	88-98
1:8	Average %	98
	Range %	93-104

回收率：

The recovery of human PRSS7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.528	2.422	2.475	2.358
10	1.746	1.692	1.719	1.602
5	1.164	1.110	1.137	1.020
2.5	0.654	0.649	0.652	0.535
1.25	0.413	0.434	0.424	0.307
0.625	0.271	0.253	0.262	0.145
0.312	0.195	0.208	0.202	0.085
0	0.120	0.114	0.117

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
基因功能参考文献：
1. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 106 M-1 sec-1) and DDDDR (kcat/KM = 1.89 x 107 M-1 sec-1) relative to all other enteropeptidase variants reported to date. PMID: 23436726
2. Enteropeptidase is a gene associated with a starvation human phenotype and a novel target for obesity treatment PMID: 23185382
3. Human enteropeptidase shows 10x faster kinetics compared to other animal sources but low solubility under low salt conditions. A supercharged variant of enteropeptidase light chain with increased solubility was used for crystallization. PMID: 22488687
4. Characterization of the different catalytic activity of human and bovine enteropeptidase light chains toward hydrolysis of peptides and proteins lacking tetraaspartate sequence. PMID: 22571433
5. Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification PMID: 11913964
6. Produced in enterocytes and goblet cells. Localization on brush border of cells for physiological activation of digestive enzymes. In duodenal polyps and adenocarcinoma at duodenum but not in Brunner's gland adenoma. PMID: 12907431
7. Enterokinase directly cleaved proMMP-9 at the Lys65-Ser66 site. PMID: 18062964
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相关疾病：

Enterokinase deficiency (ENTKD)
亚细胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Peptidase S1 family
组织特异性：

Intestinal brush border.
数据库链接：

HGNC: 9490

OMIM: 226200

KEGG: hsa:5651

STRING: 9606.ENSP00000284885

UniGene: Hs.149473

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.