Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

中文名称：

人EGF、嗜乳蛋白和7跨膜结构域蛋白1(ELTD1)酶联免疫试剂盒
货号：

CSB-EL007630HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO定量检测人表皮生长因子（EGF）、嗜乳蛋白及ELTD1的酶联免疫试剂盒，采用双抗体夹心法原理，适用于血清、血浆、组织匀浆和细胞裂解液样本的体外科研分析。EGF作为调控细胞增殖与组织修复的关键因子，嗜乳蛋白参与乳腺发育与泌乳功能，而ELTD1作为黏附G蛋白偶联受体家族成员，在血管生成及肿瘤微环境中发挥重要作用，三者均为细胞信号转导研究的热点靶标。试剂盒检测灵敏度范围为25-1600 pg/mL，可精准捕捉样本中微量目标蛋白的动态变化。其高特异性双抗体识别系统有效排除交叉反应干扰，配套标准品及优化反应体系确保实验数据的稳定性和重复性。该产品适用于基础医学研究中相关蛋白的表达水平检测，例如细胞功能调控机制探索、疾病动物模型分析或生物标志物筛选等实验场景，为肿瘤生物学、再生医学及代谢疾病研究提供可靠工具。
别名：

ADGRL4; ELTD1; ETL; UNQ202/PRO228; Adhesion G protein-coupled receptor L4; EGF, latrophilin and seven transmembrane domain-containing protein 1; EGF-TM7-latrophilin-related protein; ETL protein
缩写：

ELTD1
Uniprot No.：

Q9HBW9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	88
	Range %	85-92

回收率：

The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	87	82-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.116	2.231	2.174	2.012
800	1.597	1.643	1.620	1.458
400	1.166	1.187	1.177	1.015
200	0.833	0.826	0.830	0.668
100	0.551	0.563	0.557	0.395
50	0.362	0.372	0.367	0.205
25	0.281	0.287	0.284	0.122
0	0.161	0.162	0.162

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Endothelial orphan receptor that acts as a key regulator of angiogenesis.
基因功能参考文献：
1. High ELTD1 expression is associated with glioma. PMID: 27416955
2. miR-139-5p suppresses glioblastoma multiforme cell proliferation by targeting ELTD1 and regulating cell cycle. PMID: 26449464
3. Results provide insight into the regulation of tumor angiogenesis and highlight ELTD1 as key player in blood vessel formation. PMID: 23871637
4. Two of these (D1S0818i-ELTD1 and D6S0035i-MAPK14) remain associated following application of Bonferroni's correction and multivariate analysis. PMID: 23474535
5. Results of this study strongly suggests that associative analysis was able to accurately identify ELTD1 as a putative glioma-associated biomarker PMID: 23096411
6. Genes such as ELTD1 on chromosome 1, in addition to genes on chromosomes 4 (eg, GABRA2) and 6 (eg, CNR1), may be associated with the genetic risk for cannabis use disorders. PMID: 18519829
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亚细胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
组织特异性：

Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
数据库链接：

HGNC: 20822

OMIM: 616419

KEGG: hsa:64123

STRING: 9606.ENSP00000359778

UniGene: Hs.132314

Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

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