Human Disintegrin and metalloproteinase domain-containing protein 2(ADAM2) ELISA kit

Human Disintegrin and metalloproteinase domain-containing protein 2(ADAM2) ELISA kit

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中文名称：

人解整合素样金属蛋白酶2(ADAM2)酶联免疫试剂盒
货号：

CSB-EL001280HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人解整合素样金属蛋白酶2（ADAM2）酶联免疫试剂盒（货号：CSB-EL001280HU）是一款基于双抗体夹心法原理设计的定量检测试剂盒，适用于人血清、血浆及组织匀浆样本中ADAM2蛋白的精准分析。ADAM2是ADAM跨膜蛋白家族成员，参与细胞黏附、信号转导及蛋白水解等过程，在生殖发育及组织重塑中具有潜在调控作用。本试剂盒检测灵敏度为0.312 ng/ml，线性范围为0.312-20 ng/ml，可稳定检测低丰度样本，操作流程标准化且重复性优异。其核心组分包含预包被特异性捕获抗体的酶标板、生物素标记检测抗体及链霉亲和素-HRP系统，通过显色反应实现目标蛋白的定量分析。该产品广泛应用于生殖生物学研究、细胞迁移与分化机制探索、疾病模型构建等领域，尤其适用于探究ADAM2在精子发生、受精过程或肿瘤微环境中的功能调控。科研人员可通过该试剂盒高效完成样本中ADAM2的动态监测，为相关基础研究提供可靠数据支持。
别名：

A disintegrin and metalloproteinase domain 2 ELISA Kit; ADAM 2 ELISA Kit; ADAM metallopeptidase domain 2 ELISA Kit; Adam2 ELISA Kit; ADAM2_HUMAN ELISA Kit; AI323749 ELISA Kit; Cancer/testis antigen 15 ELISA Kit; CRYN1 ELISA Kit; CRYN2 ELISA Kit; CT15 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 2 ELISA Kit; Fertilin beta ELISA Kit; Fertilin subunit beta ELISA Kit; Ftnb ELISA Kit; PH 30 ELISA Kit; PH 30b ELISA Kit; PH-30 ELISA Kit; PH30 ELISA Kit; Ph30 beta ELISA Kit; PH30-beta ELISA Kit
缩写：

ADAM2
Uniprot No.：

Q99965
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/ml - 20 ng/ml
灵敏度：

0.078 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	97-105
1:2	Average %	106
	Range %	102-111
1:4	Average %	90
	Range %	86-96
1:8	Average %	97
	Range %	90-104

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.305

2.234

2.269

2.100

1.989

1.967

1.978

1.809

1.447

1.388

1.418

1.248

2.5

0.788

0.779

0.783

0.614

1.25

0.433

0.421

0.427

0.258

0.625

0.330

0.323

0.327

0.157

0.312

0.266

0.274

0.270

0.101

0.173

0.165

0.169

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
基因功能参考文献：
1. ADAM2, CALR3 and SAGE1 cancer/testis antigens are not promising targets for immunotherapy of breast and lung cancer. PMID: 26252478
2. The fertilin beta contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
亚细胞定位：

Membrane; Single-pass type I membrane protein.
组织特异性：

Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
数据库链接：

HGNC: 198

OMIM: 601533

KEGG: hsa:2515

STRING: 9606.ENSP00000265708

UniGene: Hs.177959

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.