Human Desert hedgehog (DHH) ELISA kit

Human Desert hedgehog (DHH) ELISA kit

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中文名称：

人沙漠刺猬因子(DHH)酶联免疫试剂盒
货号：

CSB-E12006h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒用于定量检测人沙漠刺猬因子（Desert Hedgehog, DHH）蛋白，该靶点属于Hedgehog信号通路家族成员，在神经组织发育、细胞分化和生殖系统调控中发挥重要作用，其异常表达可能与神经性疾病及生殖系统功能紊乱相关。本产品采用双抗体夹心酶联免疫吸附法（Sandwich ELISA），特异性识别DHH蛋白的天然构象，覆盖15.6 pg/mL至1000 pg/mL的宽线性范围，最低检测限达7.8 pg/mL，适用于血清、血浆、细胞培养上清及组织匀浆等样本类型的检测。实验操作全程可在4-5小时内完成，试剂盒包含预包被96孔板、标准品、生物素标记检测抗体、链霉亲和素-HRP及显色底物等核心组分，支持科研领域对DHH蛋白表达水平的精准分析，例如基础研究中信号通路机制探索、疾病模型中DHH动态变化的系统性研究，或在药物开发过程中评估干预措施对Hedgehog通路的影响。本产品严格进行批次间质控，确保实验数据可靠性和重复性，仅限科学研究使用。
别名：

C78960 ELISA Kit; Desert hedgehog (Drosophila) homolog ELISA Kit; Desert hedgehog ELISA Kit; Desert hedgehog homolog (Drosophila) ELISA Kit; Desert hedgehog protein C-product ELISA Kit; Desert hedgehog protein precursor ELISA Kit; DHH ELISA Kit; DHH_HUMAN ELISA Kit; GDXYM ELISA Kit; HHG-3 ELISA Kit; Hira ELISA Kit; SRXY7 ELISA Kit
缩写：

DHH
Uniprot No.：

O43323
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Stem Cells
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	84-92
1:10	Average %	99
	Range %	95-101
1:20	Average %	99
	Range %	95-102
1:40	Average %	95
	Range %	90-98

回收率：

The recovery of human DHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-96
EDTA plasma (n=4)	96	94-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.418	2.402	2.410	2.297
500	1.766	1.880	1.823	1.710
250	1.223	1.213	1.218	1.105
125	0.689	0.707	0.698	0.585
62.5	0.453	0.432	0.443	0.330
31.2	0.324	0.331	0.328	0.215
15.6	0.224	0.218	0.221	0.108
0	0.115	0.111	0.113

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Expression of Hedgehog Pathway Mediator GLI Represents a Negative Prognostic Marker in Human Acute Myeloid Leukemia and Its Inhibition Exerts Antileukemic Effects. Wellbrock J.et al,Clin Cancer Res.,2015

功能：

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
基因功能参考文献：
1. Whole-exome sequencing revealed a homozygous variant in DHH leading to the p.Trp173Cys substitution. The relevant Trp residue is conserved, and its alteration was predicted to be deleterious. Molecular dynamics simulations showed that the mutation increases the conformational flexibility of the protein PMID: 28708305
2. Single nucleotide polymorphism in the DHH gene is associated with bipolar disorder. PMID: 25517604
3. Mutations in DHH play a role in 46,XY gonadal dysgenesis and are associated with seminoma formation and a neuropathy with minifascicle formation. PMID: 25927242
4. Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
5. Mutations in DHH are associated with 46,XY pure gonadal dysgenesis and mixed gonadal dysgenesis. PMID: 23786321
6. This study demonistrated that Desert hedgehog links transcription factor Sox10 to peripheral nerve development. PMID: 22514309
7. Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
8. Two cases have been described in Indian patients with 46,XY complete gonadal dysgenesis that could be attributable to mutations in the Desert hedgehog (DHH) gene leading to non-functional DHH protein. PMID: 21816240
9. We found no significant correlation between the expression of SOX9 and desert hedgehog, and neither SOX9 nor desert hedgehog expression was correlated to the histoprognostic grade in sarcomas. PMID: 21193222
10. These data demonstrate that DHH is a key molecule in both male gonadal differentiation and perineurial formation in peripheral nerves PMID: 11990454
11. Importance of DHH in mammalian male sexual differentiation, providing extended evidence that DHH constitutes a key gene in gonadal differentiation PMID: 16390857
12. its signal transduction regulates tumor development. (review) PMID: 18788453
13. Hh signaling is important in the pathogenesis of B-CLL and, hence, may be a potential therapeutic target PMID: 19074837
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相关疾病：

Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD); 46,XY sex reversal 7 (SRXY7)
亚细胞定位：

[Desert hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Desert hedgehog protein C-product]: Secreted, extracellular space.
蛋白家族：

Hedgehog family
数据库链接：

HGNC: 2865

OMIM: 233420

KEGG: hsa:50846

STRING: 9606.ENSP00000266991

UniGene: Hs.524382

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.