Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

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中文名称：

人D-乳酸脱氢酶(D-LDH)酶联免疫试剂盒
货号：

CSB-E11720h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人D-乳酸脱氢酶（D-LDH）酶联免疫定量检测试剂盒（货号：CSB-E11720h），采用双抗体夹心法原理设计，可精准检测血清、血浆及组织匀浆样本中D-LDH的浓度水平。D-LDH是催化D-乳酸氧化代谢的关键酶，其活性变化与肠道菌群代谢、缺氧状态及某些代谢性疾病密切相关。试剂盒检测线性范围覆盖31.25至2000 mIU/mL，具备高灵敏度和特异性，样本仅需常规离心预处理即可快速完成检测，适用于科研领域中对D-LDH表达的定量分析。该产品可广泛应用于肠道功能研究、代谢通路探索、疾病模型构建等方向，例如评估缺血再灌注损伤、肠道屏障功能障碍或微生物代谢研究中D-LDH的动态变化，为能量代谢调控机制及病理生理研究提供可靠工具。试剂盒包含预包被酶标板、标准品、检测抗体及显色系统等完整组分，操作流程标准化，支持批量样本的高效检测需求。
别名：

D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
缩写：

LDHD
Uniprot No.：

Q86WU2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 mIU/mL-2000 mIU/mL
灵敏度：

7.8 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-103
1:2	Average %	91
	Range %	86-103
1:4	Average %	94
	Range %	86-105
1:8	Average %	91
	Range %	84-100

回收率：

The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-106
EDTA plasma (n=4)	96	86-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
2000	2.418	2.385	2.402	2.265
1000	1.773	1.728	1.751	1.614
500	0.936	1.002	0.969	0.832
250	0.624	0.651	0.638	0.501
125	0.407	0.422	0.415	0.278
62.5	0.282	0.287	0.285	0.148
31.25	0.253	0.264	0.259	0.122
0	0.135	0.139	0.137

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

PPARA ameliorates sepsis-induced myocardial injury via promoting macrophage M2 polarization by interacting with DUSP1 L Cheng,Regenerative therapy,2024
Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells Shi S.et al,Mol Med Rep,2017

功能：

Involved in D-lactate, but not L-lactate catabolic process.
基因功能参考文献：
1. The activity and expression of the mitochondrial D-lactate dehydrogenase, is higher in prostate cancer versus normal prostate cells. PMID: 23333299
2. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
3. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
亚细胞定位：

Mitochondrion.
蛋白家族：

FAD-binding oxidoreductase/transferase type 4 family
组织特异性：

Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney.
数据库链接：

HGNC: 19708

OMIM: 607490

KEGG: hsa:197257

STRING: 9606.ENSP00000300051

UniGene: Hs.380929

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.