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  4. Human Cytokine-like protein 1(CYTL1) ELISA kit

Human Cytokine-like protein 1(CYTL1) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    人细胞因子样蛋白1(CYTL1)酶联免疫试剂盒
  • 货号：
    CSB-EL006472HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人细胞因子样蛋白1(CYTL1)酶联免疫试剂盒（CSB-EL006472HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYTL1含量。CYTL1是一种小分子量的分泌蛋白，最初在CD34+细胞中发现。研究发现，CYTL1在细胞内具有新型抑癌因子特征，通过稳定NDUFV1抑制乳腺癌代谢重编程，其作用机制涉及与MDM2竞争性结合在NDUFV1的1-80结构域，从而干扰NDUFV1的泛素化降解，实现稳态的OXPHOS代谢。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可广泛应用于细胞因子调控机制研究、疾病动物模型中的蛋白表达动态分析，或体外培养细胞上清液中CYTL1分泌水平的定量评估，为探索其生物学功能及信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C17 ELISA Kit; C4orf4 ELISA Kit; CYTL1 ELISA Kit; CYTL1_HUMAN ELISA Kit; Cytokine like 1 ELISA Kit; Cytokine like protein C17 ELISA Kit; Cytokine-like protein 1 ELISA Kit; Protein C17 ELISA Kit
  • 缩写：
    CYTL1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 82-91    
    1:2 Average % 94    
    Range % 89-98    
    1:4 Average % 94    
    Range % 90-97    
    1:8 Average % 92    
    Range % 88-97    
  • 回收率：
    The recovery of human CYTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 91-99    
    EDTA plasma (n=4) 103 98-108    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.008 2.102 2.055 1.877    
    5 1.735 1.797 1.766 1.588    
    2.5 1.408 1.369 1.389 1.211    
    1.25 1.121 1.068 1.095 0.917    
    0.625 0.737 0.728 0.733 0.555    
    0.312 0.488 0.468 0.478 0.300    
    0.156 0.336 0.331 0.334 0.156    
    0 0.178 0.178 0.178 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CYTL1 antibodies

CYTL1 Proteins

靶点详情

  • 基因功能参考文献：
    1. Circular dichroism analysis showed that, like chemokines, CYTL1has a higher content of beta-sheet than alpha-helix secondary structure; recombinant CYTL1 promoted calcium flux in chondrocytes; unlike chemokines, CYTL1 had limited affinity to proteoglycans; together, these properties further support cytokine-like properties for CYTL1 with some overlap with the chemokines PMID: 28478073
    2. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
    3. when the native signal peptide of CYTL1 was replaced by IgGkappaSP, the expression and secretion was increased by 4 fold. PMID: 26922322
    4. role in regulation embryo implantation PMID: 26800213
    5. Cytl1 is highly expressed in the endometrium during the embryo implantation and its expression is regulated by ovarian hormones. Cytl1 enhances endometrial proliferation, induces the secretion of endometrial LIF and HB-EGF, and even enhances endometrial cell adhesion to trophoblastic cells. These indicate that Cytl1 is a potential molecular mediator of embryo is a potential molecular mediator in embryo implantation. PMID: 26800213
    6. our results showed the first evidence of CYTL1 expression in SH-SY5Y neuroblastoma cells and human NB tissues, revealed a possible link between CYTL1 and NB development PMID: 22797702
    7. evidence suggests that ANKRD7 and CYTL1 genes may play an important role in the variance in alcohol drinking risk PMID: 22613542
    8. C17orf62-L could induce cell death accompanied with rising of cleaved PARP. PMID: 21503106
    9. The DNA methylation pattern of the CYTL1 promoter region was significantly different between early and advanced stages of squamous cell carcinoma. PMID: 22011669
    10. C17 as a cytokine that s contributes to immune homeostasis systemically or in a tissue-specific manner in the joint PMID: 21799806
    11. We report a structural-functional characterization of cytokine-like protein 1 (Cytl1) by a combination of different computational structure-based techniques. PMID: 21322034

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Specifically expressed in CD34+ hematopoietic cells.
  • 数据库链接：

    HGNC: 24435

    OMIM: 607930

    KEGG: hsa:54360

    STRING: 9606.ENSP00000303550

    UniGene: Hs.13872

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |