Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Cystatin-SN(CST1) ELISA kit

Human Cystatin-SN(CST1) ELISA kit

文献引用 (9) 说明书下载
  • 中文名称：
    人胱抑素SN(CST1)酶联免疫试剂盒
  • 货号：
    CSB-EL006088HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人胱抑素SN（CST1）酶联免疫试剂盒（货号：CSB-EL006088HU），基于双抗体夹心法定量检测样本中CST1蛋白含量，适用于血清、血浆、细胞培养上清、尿液、组织匀浆及细胞裂解液等多种样本类型。CST1属于胱抑素超家族成员，在黏膜分泌物中广泛表达，参与调控炎症反应及肿瘤微环境，其表达水平与呼吸道、消化道疾病及某些上皮源性肿瘤的病理进程相关。该试剂盒检测灵敏度为0.156 ng/mL，线性检测范围为0.156-10 ng/mL，可精准检测低丰度目标蛋白，操作流程约需3.5小时完成。科研领域可用于探究CST1在黏膜防御机制、炎症相关信号通路或肿瘤微环境中的生物学功能，也可作为研究慢性炎症性疾病、黏膜屏障功能障碍或肿瘤标志物筛选的工具，为疾病机制研究提供数据支持。试剂盒包含预包被捕获抗体、检测抗体、标准品及显色系统，支持96孔板规格，需自备酶标仪等基础实验设备。
  • 别名：
    CST1 ELISA Kit; Cystain SAI ELISA Kit; Cystain-SA-I ELISA Kit; Cystatin 1 ELISA Kit; Cystatin-1 ELISA Kit; Cystatin-SN ELISA Kit; Cystatin1 ELISA Kit; Cysteine proteinase inhibitor, type 2 family ELISA Kit; CYTN ELISA Kit; CYTN_HUMAN ELISA Kit; Salivary cystatin SA1 ELISA Kit; Salivary cystatin-SA-1 ELISA Kit
  • 缩写：
    CST1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates,urine, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CST1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 92-97  
    1:2 Average % 100  
    Range % 98-104  
    1:4 Average % 97  
    Range % 92-101  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human CST1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 93 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.406 2.389 2.398 2.278  
    5 1.841 1.776 1.809 1.689  
    2.5 1.246 1.223 1.235 1.115  
    1.25 0.695 0.685 0.690 0.570  
    0.625 0.464 0.444 0.454 0.334  
    0.312 0.252 0.249 0.251 0.131  
    0.156 0.187 0.185 0.186 0.066  
    0 0.122 0.118 0.120 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

CST1 antibodies

CST1 Proteins

靶点详情

  • 功能：
    Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
  • 基因功能参考文献：
    1. Results revealed that CST1 expression was upregulated in colon cancer (CC) compared with normal tissues and contributed to CC cell proliferation. PMID: 29845224
    2. High CST1 expression is negatively correlated with survival of breast cancer patients. CST1 promotes cell proliferation, clone formation, and metastasis in breast cancer cells. CST1 is a novel potential prognostic biomarker and therapeutic target for breast cancer. PMID: 28523467
    3. this results identified cystatin 1 as a biomarker in patients with seasonal allergic rhinitis PMID: 28633877
    4. These results indicate that CST1-mediated extracellular CatB activity enhances tumor development by preventing cellular senescence. PMID: 28383558
    5. interactions of human family 1 & 2 cystatins with cathepsin L1 PMID: 27764212
    6. high expression of Cystatin SN is a significant prognostic indicator of a higher rate of recurrence, metastatic risk, and poor survival in patients with surgically resected NSCLCs. PMID: 25648368
    7. In conclusion, our data suggest that CST1 might contribute to the proliferation of pancreatic cancer cells and could be a potential biomarker for the early detection of pancreatic cancer. PMID: 25577248
    8. CST1 may contribute to inactivation of protease allergens and help re-establish homeostasis of the nasal membranes. PMID: 23950865
    9. CST1 expression at both the messenger RNA and protein levels was barely detected in control cells, which included early passage proliferating, quiescent, or immortal human fibroblasts PMID: 21636832
    10. Murine monoclonal antibodies were made which can distinguish between CST1 and CST2. PMID: 15829315
    11. CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases. PMID: 19463800
    12. Identification of Cystatin SN as a novel tumor marker for colorectal cancer. PMID: 19513549

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 组织特异性：
    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
  • 数据库链接：

    HGNC: 2473

    OMIM: 123855

    KEGG: hsa:1469

    STRING: 9606.ENSP00000305731

    UniGene: Hs.123114

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |