Human Complement fragment 4b,C4b ELISA Kit

Human Complement fragment 4b,C4b ELISA Kit

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中文名称：

人补体片断4b(C4b)酶联免疫试剂盒
货号：

CSB-E09302h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人补体片断4b（C4b）酶联免疫试剂盒（货号：CSB-E09302h），基于双抗体夹心法原理设计，可对血清、血浆样本中的C4b蛋白进行定量检测，其检测灵敏度为15.6 ng/mL，线性范围覆盖15.6 ng/mL至1000 ng/mL。C4b是补体系统激活过程中产生的关键片段，参与经典和凝集素途径的免疫应答调控，在病原体清除、炎症反应及免疫复合物处理中发挥重要作用，其水平变化与自身免疫性疾病、感染性疾病及慢性炎症的病理机制研究密切相关。该试剂盒通过特异性抗体对实现高灵敏度检测，适用于科研领域中对补体系统功能的探索，例如在炎症模型、免疫调节实验或疾病机制研究中定量分析血清/血浆样本的C4b表达动态，为揭示补体介导的生理病理过程提供可靠工具。实验操作简便，仅需标准实验室设备即可完成，配套试剂包含预包被酶标板、检测抗体及标准品，支持科研人员高效开展体外样本分析。
别名：

Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit
缩写：

C4b
Uniprot No.：

P0C0L5
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-94
1:400	Average %	97
	Range %	91-102
1:800	Average %	98
	Range %	92-105
1:1600	Average %	92
	Range %	86-99

回收率：

The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	98	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.566	2.366	2.466	2.373
500	2.031	1.931	1.981	1.888
250	1.409	1.319	1.364	1.271
125	0.867	0.854	0.861	0.768
62.5	0.524	0.514	0.519	0.426
31.2	0.345	0.325	0.335	0.242
15.6	0.213	0.203	0.208	0.115
0	0.094	0.091	0.093

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intraperitoneal MASP-1 Levels are Associated with Peritoneal Solute Transport Rate in Peritoneal Dialysis Patients: A Retrospective Cohort Study J Li, Y Xiong, M Chen, D Xu, L Zhou,Journal of Inflammation Research,2024
Elevated complement mediator levels in endothelial-derived plasma exosomes implicate endothelial innate inflammation in diminished brain function of aging humans FM Elahi,Scientific Reports,2021
Traumatic brain injury increases plasma astrocyte┸\derived exosome levels of neurotoxic complement proteins Goetzl EJ,FASEB,2020
High complement levels in astrocyte-derived exosomes of Alzheimer\'s disease Goetzl EJ.et al,Ann Neurol,2018
Serum complement C4b, fibronectin, and prolidase are associated with the pathological changes of pulmonary tuberculosis Wang C et al,BMC Infect Dis,2014

功能：

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
相关疾病：

Systemic lupus erythematosus (SLE); Complement component 4B deficiency (C4BD)
亚细胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
组织特异性：

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extr
数据库链接：

HGNC: 1324

OMIM: 120820

KEGG: hsa:100293534

STRING: 9606.ENSP00000415941

UniGene: Hs.534847

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.