Human Complement fragment 3c,C3c ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人补体片段3c(C3c)酶联免疫试剂盒（CSB-E11168h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、唾液样本中的C3c含量。C3c是补体系统中的一个重要成分，参与经典和替代途径的补体激活。其在肿瘤免疫和病毒感染中发挥重要作用，如通过调节免疫反应和直接杀伤肿瘤细胞来抑制肿瘤生长，同时在HBV宫内传播中与免疫复合物相互作用。C3c的研究有助于深入理解补体系统在免疫调节和疾病发生中的作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对补体系统功能的探索，例如在炎症机制研究、疾病模型构建或药物干预实验中动态监测补体激活状态，也可用于体外培养体系中补体相关生物标志物的分析，为免疫调节研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

C3c
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine, saliva
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C3c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	88
	Range %	84-92
1:10000	Average %	94
	Range %	90-98
1:20000	Average %	99
	Range %	95-104
1:40000	Average %	102
	Range %	96-108

回收率：

The recovery of human C3c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.