Human Complement Fragment 4d,C4d ELISA Kit

产品详情

产品描述：

CUSABIO人补体片段4d（C4d）酶联免疫检测试剂盒（货号：CSB-E14192h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的C4d含量。C4d是补体活化途径的关键片段，其稳定沉积常作为组织免疫损伤的生物标志物，在移植排斥反应监测、自身免疫性疾病及炎症机制研究中具有重要价值。试剂盒检测灵敏度为0.78 ng/mL，线性范围为0.78-50 ng/mL，实验全程约需4.5小时，包含预包被特异性抗体的96孔板、标准品、检测抗体及显色试剂等全套组分，支持科研人员高效完成样本检测。该试剂盒适用于体外研究场景，如探讨补体系统在心血管疾病、肾脏病理或免疫复合物相关疾病中的作用机制，也可用于评估实验动物模型或细胞实验中补体激活水平，为免疫相关基础研究提供精准定量工具。
缩写：

C4d
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4d in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	103
	Range %	94-108
1:200	Average %	98
	Range %	90-104
1:400	Average %	89
	Range %	82-93
1:800	Average %	95
	Range %	88-98

回收率：

The recovery of human C4d spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	113	103-117
EDTA plasma (n=4)	94	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.