Human Complement C1q tumor necrosis factor-related protein 2(C1QTNF2) ELISA kit

中文名称：

人补体C1q肿瘤坏死因子相关蛋白2(C1QTNF2)酶联免疫试剂盒
货号：

CSB-EL003647HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人补体C1q肿瘤坏死因子相关蛋白2（C1QTNF2）酶联免疫检测试剂盒（货号：CSB-EL003647HU），采用双抗体夹心技术实现定量检测，适用于血清、血浆及组织匀浆样本中C1QTNF2蛋白的精准分析。C1QTNF2作为补体C1q/TNF超家族成员，在脂肪细胞分化、糖代谢调节及炎症反应中发挥重要作用，是研究代谢性疾病、慢性炎症及心血管病理机制的潜在生物标志物。该试剂盒检测灵敏度高，线性范围为0.156 ng/mL至10 ng/mL，可稳定识别天然与重组蛋白，实验操作流程经过优化，兼容常规实验室设备。科研人员可通过该试剂盒探索C1QTNF2在肥胖、糖尿病、动脉粥样硬化等疾病模型中的表达变化，或用于评估药物干预对代谢相关信号通路的影响。产品包含预包被抗体、标准品及检测所需全套试剂，支持96孔板规格，提供标准曲线参考及样本处理建议，为体外实验提供高效可靠的定量分析工具。
别名：

C1q and tumor necrosis factor related protein 2 ELISA Kit; C1QT2_HUMAN ELISA Kit; C1qtnf2 ELISA Kit; Complement C1q tumor necrosis factor-related protein 2 ELISA Kit; CTRP2 ELISA Kit; Zacrp2 ELISA Kit
缩写：

C1QTNF2
Uniprot No.：

Q9BXJ5
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	91-99
1:10	Average %	103
	Range %	98-106
1:20	Average %	110
	Range %	106-114
1:40	Average %	92
	Range %	89-97

回收率：

The recovery of human C1QTNF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	97	93-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.532	2.604	2.568	2.382
5	2.059	1.987	2.023	1.837
2.5	1.307	1.337	1.322	1.136
1.25	0.773	0.768	0.771	0.585
0.625	0.503	0.512	0.508	0.322
0.312	0.307	0.314	0.311	0.125
0.156	0.247	0.257	0.252	0.066
0	0.185	0.187	0.186

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Involved in the regulation of lipid metabolism in adipose tissue and liver.
亚细胞定位：

Secreted.
组织特异性：

Expressed in adipose tissue.
数据库链接：

HGNC: 14325

KEGG: hsa:114898

STRING: 9606.ENSP00000377545

UniGene: Hs.110062

Human Complement C1q tumor necrosis factor-related protein 2(C1QTNF2) ELISA kit

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