Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Complement 7,C7 ELISA Kit

Human Complement 7,C7 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    人补体7(C7)酶联免疫试剂盒
  • 货号：
    CSB-E11167h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人补体7（Complement Component 7, C7）是补体系统的重要组成部分，参与形成膜攻击复合物（MAC），在先天免疫防御和炎症反应中发挥关键作用，其表达异常与感染性疾病、自身免疫性疾病及某些遗传性补体缺陷密切相关。本试剂盒（货号：CSB-E11167h）采用双抗体夹心法原理，定量检测人血清、血浆或组织匀浆样本中的C7蛋白，检测灵敏度达15.6 ng/mL，线性范围为15.6-1000 ng/mL，可精准覆盖生理及病理状态下的浓度变化。该试剂盒包含预包被特异性捕获抗体的酶标板、检测抗体及优化的反应体系，操作流程标准化，适用于科研场景中探索C7在感染性疾病（如细菌/病毒感染）、自身免疫疾病（如系统性红斑狼疮）或心血管疾病（如动脉粥样硬化）中的调控机制，也可用于评估药物干预或基因修饰对补体系统的影响。实验支持多种生物样本类型，为研究补体通路功能、炎症反应及免疫相关疾病提供可靠工具。
  • 别名：
    C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit
  • 缩写：
    C7
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %91
    Range %87-95
    1:200Average %95
    Range %90-100
    1:400Average %97
    Range %94-101
    1:800Average %94
    Range %91-98
  • 回收率：
    The recovery of human C7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)9993-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.934 2.880 2.907 2.806
    5002.102 2.195 2.149 2.048
    2501.421 1.504 1.463 1.362
    1250.807 0.772 0.790 0.689
    62.50.443 0.459 0.451 0.350
    31.20.269 0.259 0.264 0.163
    15.60.171 0.167 0.169 0.068
    00.099 0.103 0.101
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

相关产品

C7 antibodies

C7 Proteins

靶点详情

  • 功能：
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
  • 基因功能参考文献：
    1. this study shows that GG genotype of C7 provides protection against fibrosis severity while showing a higher risk for hepatocellular carcinoma in patients with hepatitis C PMID: 29966690
    2. Two case reports of C7 deficiency leading to recurrent meningitis and other bacterial infections are described. PMID: 28078901
    3. The complement C7 rs6876739 CC genotypes and mannan-binding lectin (MBL2) gene polymorphisms of liver donors were significantly associated with bacterial infection in recipients. PMID: 27063552
    4. Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1 pathway. PMID: 26723877
    5. Borrelial CspA binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC). PMID: 23943762
    6. Studies indicate that the deletion defect may be a more commonly distributed cause of C7 deficiency in Ireland. PMID: 22206826
    7. C7 isoelectric focusing variants can determine meningococcal killing in the early stage of infection when antibody-independent killing prevails. PMID: 19931914
    8. To determine transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. PMID: 12595902
    9. The interaction between the factor I domain of C7 and the C345C domain at the C terminus of the C5 alpha-chain plays an essential role in complement membrane attack complex formation and complement lytic activity. PMID: 15879120
    10. recurrence of fulminant meningococcal disease in a complement component C7-deficient patient PMID: 15889368
    11. membrane associated C7 acts as a trap for the late complement components to control excessive inflammation induced by SC5b-9 PMID: 19179470
    12. C7 is associated with multiple sclerosis pathogenesis. PMID: 19221116
    13. Data show that the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. PMID: 19419965

    显示更多

    收起更多

  • 相关疾病：
    Complement component 7 deficiency (C7D)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Complement C6/C7/C8/C9 family
  • 数据库链接：

    HGNC: 1346

    OMIM: 217070

    KEGG: hsa:730

    STRING: 9606.ENSP00000322061

    UniGene: Hs.669878

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |