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  4. Human Complement 1q,C1q ELISA Kit

Human Complement 1q,C1q ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    人补体1q(C1q)酶联免疫试剂盒
  • 货号：
    CSB-E10118h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人补体1q(C1q)酶联免疫试剂盒（CSB-E10118h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QB含量。C1QB是补体系统C1复合物的重要组成部分，补体系统在免疫防御等过程中发挥关键作用。相关研究主要围绕其在补体激活机制中的功能展开，探究其与免疫相关疾病的关联、在信号传导中的作用等，旨在为疾病治疗提供新靶点和思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中C1q相关的免疫调控机制研究、疾病模型构建或药物干预效果评估等场景，例如通过检测实验动物血清或组织中的C1q浓度，探究其在炎症性疾病或神经退行性疾病中的分子作用机制，为相关基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C1qb ELISA Kit; C1QB_HUMAN ELISA Kit; Complement C1q subcomponent subunit B ELISA Kit; Complement component 1 q subcomponent B chain ELISA Kit; Complement component 1 q subcomponent beta polypeptide ELISA Kit; Complement component C1q B chain ELISA Kit; Complement subcomponent C1q chain B ELISA Kit
  • 缩写：
    C1QB
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C1q in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 103
    Range % 100-106
    1:2 Average % 99
    Range % 92-106
    1:4 Average % 89
    Range % 85-93
    1:8 Average % 94
    Range % 90-98
  • 回收率：
    The recovery of human C1q spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 102-108
    EDTA plasma (n=4) 95 90-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.015 1.952 1.984 1.859
    5 1.758 1.878 1.818 1.693
    2.5 1.514 1.569 1.542 1.417
    1.25 0.995 0.968 0.982 0.857
    0.625 0.614 0.632 0.623 0.498
    0.312 0.475 0.457 0.466 0.341
    0.156 0.334 0.355 0.345 0.220
    0 0.123 0.126 0.125 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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C1QB antibodies

C1QB Proteins

靶点详情

  • 功能：
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • 基因功能参考文献：
    1. C1QB expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    2. We identified a major linear epitope of C1q that is the target of anti-C1q in systemic lupus erythematosus. PMID: 22740328
    3. The susceptibility for schizophrenia was particularly associated with C1QB rs291982 GG genotype. PMID: 21951915
    4. a 2-gene signature consisting of PLEK2 and C1QB led to the best result that correctly classified 93.3% melanoma patients and 90% healthy controls PMID: 21698244
    5. The C-terminal globular region of the C1Q B chain may have evolved as a functionally specialized domain or module with distinct binding properties which together with the A and C chains confers versatility and flexibility to the whole C1q molecule. PMID: 12847249
    6. Complementary interacting sites on the C1q globular domain have been precisely defined. Characterization of point mutants suggests a central role for Arg114 and a complementary role for Arg129, Arg163, and His117 of C1Q B chain in the C1q-IgG interaction. PMID: 15034050
    7. The first analysis of C1q by mass spectrometry yields evidence that the B chain moiety of the globular head is involved in the interaction with fucoidan and underscores the particular role of arginine-109 in the charge pattern recognition property of C1q. PMID: 15709773
    8. results suggest that charged residues belonging to the apex of the gC1q heterotrimer (with participation of all three chains) as well as the side of the ghB are crucial for C1q binding to ligands, IgG1, C-reactive protein, and pentraxin 3. PMID: 16566583
    9. No C1q or low molecular weight C1q was detected in sera and no anti-C1q autoantibodies were found. Sequencing of the C1q genes revealed a novel missense mutation (Gly-Arg) in codon 217 of the B chain. PMID: 17513176

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  • 相关疾病：
    Complement component C1q deficiency (C1QD)
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 1242

    OMIM: 120570

    KEGG: hsa:713

    STRING: 9606.ENSP00000313967

    UniGene: Hs.8986

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |