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中文名称：人Collectrin蛋白/跨膜蛋白27(TMEM27)酶联免疫试剂盒
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货号：CSB-EL023823HU
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：人Collectrin蛋白/跨膜蛋白27(TMEM27)酶联免疫试剂盒（CSB-EL023823HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆、细胞裂解物样本中的TMEM27含量。Collectrin（TMEM27）是一种跨膜蛋白，与ACE2同源，主要在肾脏近端肾小管和集合管表达。其研究机制涉及氨基酸转运，与肥胖症、糖尿病等代谢性疾病相关。研究表明，Collectrin在维持血压稳定和调节肾脏功能中发挥重要作用。试剂盒检测范围为56 pg/mL-800 pg/mL，可为科研人员探究TMEM27在代谢调控、肾脏功能及相关疾病模型中的作用提供可靠工具。适用于体外样本检测，操作便捷且重复性良好，可广泛用于基础研究领域，如通过分析不同处理条件下细胞或动物模型中TMEM27的表达变化，揭示其在能量代谢、器官发育或病理进程中的分子机制，为相关疾病的机制探索及潜在治疗靶点研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：UNQ679 ELISA Kit; Collectrin ELISA Kit; kidney-specific membrane protein ELISA Kit; NX-17 ELISA Kit; NX17 ELISA Kit; PRO1312 ELISA Kit; Tmem27 ELISA Kit; TMM27_HUMAN ELISA Kit; Transmembrane protein 27 ELISA Kit; UNQ679/PRO1312 ELISA Kit
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缩写：TMEM27
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma,tissue homogenates, cell lysates
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检测范围：56 pg/mL-800 pg/mL
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灵敏度：24 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
800
2.715
2.852
2.784
2.734
400
1.507
1.426
1.467
1.417
280
0.861
0.834
0.848
0.798
144
0.372
0.399
0.386
0.336
56
0.160
0.153
0.157
0.107
0
0.048
0.051
0.050
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数据处理：
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货期：3-5 working days
引用文献
- Urinary Collectrin as Promising Biomarker for Acute Kidney Injury in Patients Undergoing Cardiac Surgery J Tichy,Biomedicines,2023
- Urinary Collectrin (TMEM27) as Novel Marker for Acute Kidney Injury S Pajenda,Life,2022
- Correlation of serum collectrin level and preeclampsia onset: A case control study MMJ Al-Bayati,J Gynecol Obstet Hum Reprod,2020
- New Insights into the Pathogenesis of Intradialytic Hypertension Hajal J,The Open Urology & Nephrology,2018
- Tmem27 is upregulated by vitamin D in INS-1 cells and its serum concentrations are low in patients with autoimmune diabetes Pepaj M et al,Scand J Clin Lab Invest,2014
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靶点详情
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功能：Plays an important role in amino acid transport by acting as binding partner of amino acid transporters SLC6A18 and SLC6A19, regulating their trafficking on the cell surface and their amino acid transporter activity. May also play a role in trafficking of amino acid transporters SLC3A1 and SLC7A9 to the renal cortical cell membrane. Regulator of SNARE complex function. Stimulator of beta cell replication.
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基因功能参考文献：
- Maternal serum collectrin levels are significantly lower in patients with preeclampsia than in the control group. There is an inverse correlation between serum collectrin levels and blood pressure. PMID: 28764560
- Lack of expression of the TMEM27 in conventional renal cell carcinoma defines a group of patients at high risk for cancer-related death. PMID: 27417314
- Tmem27 is present in human serum and its levels are significantly lower in subjects with autoimmune diabetes as compared to healthy individuals. PMID: 24693993
- Bace2 specifically targets Tmem27 and cleaves its extracellular domain, which is then shed from the plasma membrane of pancreatic beta cells. PMID: 21907142
- Tmem27 dimerization is a dynamic process involving Bace2 PMID: 22628310
- Collectrin and ACE2 in renal and intestinal amino acid transport. PMID: 21814048
- Data support a role for TMEM27 in glucose-induced insulin secretion but not in cell proliferation. The finding that its cleavage is not specific to beta cells challenges the current support for its use as a potential beta cell mass biomarker. PMID: 20386877
- Collectrin has a role in amino acid transpor in the kidney [review] PMID: 17693757
- the first human study of the gene TMEM27 and an attempt to shine a light on genotype-phenotype correlation in Turner syndrome patients. PMID: 19417552
- No TMEM27 gene mutations were discovered among 26 patients showing a phenotype resembling Dent's disease PMID: 19582483
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亚细胞定位：Cell membrane; Single-pass type I membrane protein.
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蛋白家族：TMEM27 family
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组织特异性：Kidney; collecting ducts. Pancreas; beta cells of islets.
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数据库链接：
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