Human Collagen, type III, alpha 1 (COL3A1)ELISA Kit
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中文名称：
人III型胶原α1(COL3A1)酶联免疫试剂盒
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货号：
CSB-E13446h
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规格：
96T/48T
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价格：
￥3600/￥2500
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其他：
产品详情
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产品描述：
CUSABIO人III型胶原α1（COL3A1）酶联免疫检测试剂盒（货号：CSB-E13446h），采用双抗体夹心法原理实现定量检测，适用于分析血清、血浆、细胞培养上清及组织匀浆等样本中COL3A1蛋白的表达水平。III型胶原是细胞外基质的关键成分，广泛参与组织修复、纤维化进程及胚胎发育等生理病理调控，其异常表达与器官纤维化、肿瘤微环境重塑等研究密切相关。本试剂盒检测灵敏度为0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，可精准检测低丰度样本，实验时需注意样本预处理（如血液样本需离心去除颗粒物，组织样本需匀浆后离心取上清）。该产品适用于基础科研领域，如纤维化疾病模型研究、肿瘤侵袭转移机制探索或干细胞分化过程中基质重塑分析，其高特异性抗体组合及优化的反应体系可确保数据可靠性，为细胞外基质动态变化研究提供标准化工具。
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别名：
Alpha 1 type III collagen ELISA Kit; Alpha1 (III) collagen ELISA Kit; CO3A1_HUMAN ELISA Kit; COL 3A1 ELISA Kit; COL3A1 ELISA Kit; Collagen alpha 1(III) chain ELISA Kit; Collagen alpha-1(III) chain ELISA Kit; Collagen III alpha 1 chain precursor ELISA Kit; Collagen III alpha 1 polypeptide ELISA Kit; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) ELISA Kit; Collagen type III alpha 1 ELISA Kit; Collagen type III alpha 1 chain ELISA Kit; Collagen type III alpha ELISA Kit; Collagen; fetal ELISA Kit; EDS4A ELISA Kit; Ehlers Danlos syndrome type IV; autosomal dominant ELISA Kit; Fetal collagen ELISA Kit; Type III collagen ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Homo sapiens (Human)
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样本类型：
serum, plasma, cell culture supernates, tissue homogenates
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检测范围：
0.156 ng/mL-10 ng/mL
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灵敏度：
0.039 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Signal Transduction
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:4 |
Average % |
101 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-107 |
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回收率：
| The recovery of human COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-98 |
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| EDTA plasma (n=4) |
109 |
102-115 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.484 |
2.575 |
2.530 |
2.381 |
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| 5 |
1.788 |
1.889 |
1.839 |
1.690 |
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| 2.5 |
1.256 |
1.365 |
1.311 |
1.162 |
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| 1.25 |
0.732 |
0.766 |
0.749 |
0.600 |
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| 0.625 |
0.417 |
0.450 |
0.434 |
0.285 |
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| 0.312 |
0.274 |
0.284 |
0.279 |
0.130 |
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| 0.156 |
0.248 |
0.250 |
0.249 |
0.100 |
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| 0 |
0.146 |
0.151 |
0.149 |
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本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human COL3A1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled COL3A1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：
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货期：
3-5 working days
引用文献
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Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen secretion
R Bansal,JCI insight,2024
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Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen
R Bansal,JCI insight,2024
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A Method for Simultaneous Quantification of Various Collagens by Using Nano-Liquid Chromatography Tandem Mass Spectrometry
A GONG,/,2023
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Acetylcholine decreases formation of myofibroblasts and excessive extracellular matrix production in an in vitro human corneal fibrosis model
M S?oniecka,J Cell Mol Med.,2020
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Substance P induces fibrotic changes through activation of the RhoA/ROCK pathway in an in vitro human corneal fibrosis model
Sloniecka M, et al,Journal of Molecular Medicine,2019
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Evaluation of cultured human dermal- and dermo-epidermal substitutes focusing on extracellular matrix components: Comparison of protein and RNA analysis
Corien Oostendorp.et al,Burns,2016
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Asiaticoside induction for cell-cycle progression, proliferation and collagen synthesis in human dermal fibroblasts.
Lu L.et al,Int J Dermatol.,2015
产品评价
样品类型：血清
样品信息：人
稀释比：其他 1:10
产品评价： 我用human collagen type3a1, ELISA试剂盒检测人类血清，正常、模型和给药组样本数据稳定，复孔差异小，试剂盒操作方便。另外发现该试剂盒同样可以检测到人源细胞培基上清中的col3，但各孔间的细胞数量等因素需自行把握。
靶点详情
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功能：
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
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基因功能参考文献：
- Elevated circulating PIIINP levels are associated with type 2 diabetes mellitus individuals with adipose tissue expansion and systemic proinflammatory profile suggestive for adipose tissue dysfunction. PMID: 29471595
- Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
- a variant of COL3A1 (rs3134646) is associated with the risk of developing colonic diverticulosis in white men, whereas rs1800255 (COL3A1) and rs1800012 (COL1A1) were not associated with this condition after adjusting for confounding factors. PMID: 29533249
- Brain MRI in the affected siblings as well as in the two previously reported individuals with bi-allelic COL3A1 mutations showed a brain phenotype similar to that associated with mutations in GPR56. PMID: 28258187
- Given the high specificity of the polymorphism at the rs1800255 locus of the COL3A1 gene, determined by the Sanger sequencing, it can be concluded that there is an association between this polymorphism and urinary incontinence and pelvic organ prolapse in women. PMID: 29376591
- Case Report: novel missense COL3A1 mutation in vascular Ehlers-Danlos syndrome patient presenting with pulmonary complications and iliac arterial dissection. PMID: 29216800
- Kaplan-Meir analysis of GSE7696 indicate that COL3A1 and SNAP91 correlated with survival. PMID: 27655637
- provide a picture of the gene expression changes in vascular Ehlers-Danlos syndrome skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of several structural proteins crucial to the integrity of soft connective tissues PMID: 29346445
- We identified biallelic COL3A1 variants in two unrelated families. In a 3-year-old female with developmental delay the nonsense variant c.1282C>T, p.(Arg428*) was detected in combination the c.2057delC, p.(Pro686Leufs*105) frame shift variant. PMID: 28742248
- In conclusion, based on serological collagen formation and degradation makers, penetrating Crohn's disease is associated with increased matrix metalloproteinase-9 mediated breakdown of type III collagen PMID: 28481042
- Col3A may be a potential adjunct marker for both differentiating fibroadenoma from phyllodes tumor and assessing malignant potential in PTs. PMID: 27498063
- Case Report: pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp in Ehlers-Danlos syndrome vascular type with different phenotypes in the same family. PMID: 28183226
- von Willebrand factor A1 domain-collagen binding is independent of gain- or loss-of-function phenotype and under shear stress, platelet translocation pause times on collagen-bound A1A2A3 are either normal or shorter depending on whether A1 is concertedly bound with the A3 domain to collagen. PMID: 27889474
- High serum procollagen type III N-terminal peptide expression is associated with non-alcoholic fatty liver disease. PMID: 27442361
- The findings indicate the critical role of CatB in regulating the expression of collagens III and IV by fibroblasts via prolonging TLR2/NF-kappaB activation and oxidative stress. PMID: 27648120
- High COL3A1 expression is associated with colorectal carcinoma. PMID: 26741506
- novel missense mutation c.2176G>C in Chinese family with vascular Ehlers-Danlos syndrome PMID: 25420629
- High serum Collagen Type III is associated with ovarian and breast cancer. PMID: 26406420
- A novel missense mutation in COL3A1 was found in a young patient with cervical artery dissection as the single manifestation of Ehlers-Danlos syndrome. PMID: 26497932
- the clinical phenotype of Ehlers-Danlos syndrome patients is influenced by the type of COL3A1 variant. PMID: 25758994
- Report dysregulated expression of COL3A1 in disc degeneration. PMID: 25893343
- TGFbeta target genes including TGFBI, BAMBI, COL3A1 and SERPINE1 are significantly increased in Diamond Blackfan Anemia induced pluripotent stem cells PMID: 26258650
- The production and purification of rhCOL3A1 described in this study offer a new method for obtaining high level of rhCOL3A1 in relatively pure form, which is suitable for biomedical materials application. PMID: 25231012
- These results suggest that let-7d may suppress renal cell carcinoma growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7. PMID: 25193015
- compare reported phenotypes for patients with missense variants in the C-propeptide domain for other human collagen disorders including COL1A1 and COL1A2 (osteogenesis imperfecta). PMID: 25846194
- miR-29a and miR-29b enhance cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. PMID: 25786138
- Data indicate that N-terminal propeptide of type III procollagen (PIIINP) is a highly effective means to evaluate left ventricular (LV) end-diastolic pressure (EDP) in patients with acute coronary syndrome (ACS). PMID: 25559610
- The first main finding of this study was that the rare COL3A1 rs1800255 AA genotype was associated with increased risk of anterior cruciate ligament injuries in the Polish cohort but not the South African cohort. PMID: 25073002
- During heart valve development, Krox20-mediated activation of fibrillar Col1a1 and Col3a1 genes is crucial to avoid postnatal degeneration of the aortic valve leaflets. PMID: 25344368
- In familial AAA we found one pathogenic and segregating variant (COL3A1 p.Arg491X), one likely pathogenic and segregating (MYH11 p.Arg254Cys), and fifteen VUS. PMID: 26017485
- rs1800255, COL3A1 2209 G>A polymorphism not associated with pelvic organ prolapse in Dutch population PMID: 24760181
- Variants of the COL3A1 gene are associated with a risk of stroke recurrence and prognosis. PMID: 24664438
- As a result of the translocations, COL3A1-PLAG1, the constitutively active promoter of the partner gene drives the ectopic expression of PLAG1. PMID: 24700772
- Gal-1 decreased the expression of collagen genes COL3A1 and COL5A1 but increased the expression of fibronectin and laminin 5. PMID: 24503541
- Arterial pathology in vascular Ehlers-Danlos syndrome individuals is related to the underlying COL3A1 mutation type. PMID: 24650746
- Expression of COL3A1 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. PMID: 24641356
- The results point to the fact that the injury and/or mandible fracture increase the collagen type III metabolism and its dynamics depends on the type of the used bone fixation. PMID: 24421219
- Case Report: Ehlers-Danlos syndrome IV due to a mutation in intron 14 of the COL3A1 gene leading to venous manifestations without affecting arterial vessels at clinical presentation. PMID: 23688910
- Data suggest that cardiac fibrosis, as assessed by serum extracellular matrix protein biomarkers (including PIIINP, procollagen Type III-N-terminal peptide), develops early in hypertensive patients and is predictive of cardiovascular events or death. PMID: 22788708
- analysis of collagen folding in Vascular Ehlers-Danlos syndrome mutations PMID: 23645670
- COL3A1 rs1800255, COL6A1 rs35796750 and COL12A1 rs970547 were not significantly associated with sit-and-reach, straight leg raise or total shoulder rotation range of motion PMID: 23013106
- This study indicates that SP, mediated via NK-1 R, increases collagen remodeling and leads to increased MMP3 mRNA and protein expression that is further enhanced by cyclic mechanical loading. PMID: 22836729
- IL-17, IL-23 and PIIINP have an inverse correlation with vitamin D in their involvement in the immune response in patients with HCV-4-related liver diseases in Egypt PMID: 22851868
- study found that allele A of SNP rs1800255 conferred a 1.71-fold increased risk for intracranial aneurysms (IAs) and results in an amino acid change of Ala698Thr, which led to a lower thermal stability of the peptide; results support the view that the functional variant of COL3A1 is genetic risk factors for IAs in the Chinese population PMID: 22241462
- The minimum type III sequence necessary for cleavage by the MMP1 and MMP13 was 5 GXY triplets, including 4 residues before and 11 residues after the cleavage site (P4-P11'). PMID: 22573319
- Alveolar macrophages exposed to SiO2 can induce elevated type III procollagen and collagen III expression levels in human lung fibroblasts. PMID: 21941774
- Through alveolar macrophage mediation SiO2 can accelerate the expression of TIMP-1 and collagen III, and inhibit the expression of MMP-1 in human lung fibroblasts. PMID: 20039537
- procollagen III N-terminal peptide has a role in HBeAg loss in patients with chronic hepatitis B during entecavir therapy PMID: 22019950
- Disease-associated mutations prevent GPR56-collagen III interaction. PMID: 22238662
- Data show that homozygous and compound heterozygous changes found in PLOD1 and SLC2A10 may confer autosomal recessive effects, and three MYH11, ACTA2 and COL3A1 heterozygous variants were considered as putative pathogenic gene alterations. PMID: 22001912
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相关疾病：
Ehlers-Danlos syndrome 3 (EDS3); Ehlers-Danlos syndrome 4 (EDS4)
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亚细胞定位：
Secreted, extracellular space, extracellular matrix.
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蛋白家族：
Fibrillar collagen family
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数据库链接：
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